Oligosaccharide modification by swainsonine treatment inhibits pulmonary colonization by B16-F10 murine melanoma cells

Journal Article · Sat Mar 01 00:00:00 EST 1986 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.83.6.1752· OSTI ID:5878767

Humphries, M J; Matsumoto, K; White, S L; Olden, K

Oligosaccharide moieties of cell-surface glycoconjugates are thought to be involved in recognition events associated with tumor metastasis and invasion. Using swainsonine (SW), an inhibitor of Golgi ..cap alpha..-mannosidase II that results in the formation of hybrid-type oligosaccharides on N-linked glycoproteins, the authors have tested the hypothesis that specific glycan structures are required for pulmonary colonization by tumor cells. B16-F10 murine melanoma cells were treated with SW in growth medium and then injected intravenously into syngeneic C57BL/6 mice. This treatment resulted in dramatic inhibition of colonization, but it had no effect on B16-F10 viability or on cellular tumorigenicity after subcutaneous implantation. SW-treated radiolabeled B16-F10 cells were cleared from the lungs at a great rate than control cells, suggesting that one effect of treatment is to alter tumor cell retention in the target organ. The results implicate specific glycan structures in pulmonary colonization and offer a potential approach for identification of specific macromolecules involved in tumor cell-organ recognition during metastasis.

• Research Organization: Howard Univ. Cancer Center, Washington, DC
• OSTI ID: 5878767
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 83:6
• Country of Publication: United States
• Language: English

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