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Title: Multiple forms of maize endosperm ADP-glucose pyrophosphorylase and their control by shrunken-2 and brittle-2

Abstract

Heat-labile and heat stable forms of ADP-glucose pyrophosphorylase were identified in the maize endosperm. The heat-labile form is destroyed by normal electrophoretic conditions. The heat-stable form corresponds to pyrophosphorylase B. In wild type, 96% of the total activity is heat labile. Both forms are reduced in 11 brittle-2 (bt2) and 12 shrunken-2 (sh2) mutants. The heat-labile form is reduced to a greater extent than is the heat-stable form in each of the 23 mutants. Deletion of sh2 abolishes both forms. The original ratio of the two forms is restored after sh2 function is expressed via transposition of Dissociation from sh2. The possible roles of these genes in the control of ADP-glucose pyrophosphorylase are discussed.

Authors:
; ;
Publication Date:
OSTI Identifier:
5874230
Alternate Identifier(s):
OSTI ID: 5874230
Resource Type:
Journal Article
Journal Name:
Genetics; (United States)
Additional Journal Information:
Journal Volume: 95
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PHOSPHOTRANSFERASES; STRUCTURAL CHEMICAL ANALYSIS; CHROMOSOMES; COMPARATIVE EVALUATIONS; ELECTROPHORESIS; ENDOSPERM; GENES; MAIZE; MUTANTS; QUANTITATIVE CHEMICAL ANALYSIS; TEMPERATURE EFFECTS; BODY; CEREALS; CHEMICAL ANALYSIS; ENZYMES; GRAMINEAE; GRASS; PHOSPHORUS-GROUP TRANSFERASES; PLANT TISSUES; PLANTS; TISSUES; TRANSFERASES 550200* -- Biochemistry; 550400 -- Genetics

Citation Formats

Hannah, L.C., Tuschall, D.M., and Mans, R.J. Multiple forms of maize endosperm ADP-glucose pyrophosphorylase and their control by shrunken-2 and brittle-2. United States: N. p., 1980. Web.
Hannah, L.C., Tuschall, D.M., & Mans, R.J. Multiple forms of maize endosperm ADP-glucose pyrophosphorylase and their control by shrunken-2 and brittle-2. United States.
Hannah, L.C., Tuschall, D.M., and Mans, R.J. Fri . "Multiple forms of maize endosperm ADP-glucose pyrophosphorylase and their control by shrunken-2 and brittle-2". United States.
@article{osti_5874230,
title = {Multiple forms of maize endosperm ADP-glucose pyrophosphorylase and their control by shrunken-2 and brittle-2},
author = {Hannah, L.C. and Tuschall, D.M. and Mans, R.J.},
abstractNote = {Heat-labile and heat stable forms of ADP-glucose pyrophosphorylase were identified in the maize endosperm. The heat-labile form is destroyed by normal electrophoretic conditions. The heat-stable form corresponds to pyrophosphorylase B. In wild type, 96% of the total activity is heat labile. Both forms are reduced in 11 brittle-2 (bt2) and 12 shrunken-2 (sh2) mutants. The heat-labile form is reduced to a greater extent than is the heat-stable form in each of the 23 mutants. Deletion of sh2 abolishes both forms. The original ratio of the two forms is restored after sh2 function is expressed via transposition of Dissociation from sh2. The possible roles of these genes in the control of ADP-glucose pyrophosphorylase are discussed.},
doi = {},
journal = {Genetics; (United States)},
number = ,
volume = 95,
place = {United States},
year = {1980},
month = {8}
}