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Title: Molecular characterization of nosA, a Pseudomonas stutzeri gene encoding an outer membrane protein required to make copper-containing N sub 2 O reductase

Abstract

A Pseudomonas stutzeri gene (nosA) encoding an outer membrane protein was cloned into the broad-host-range vector pRK290 and expressed in a mutant lacking the protein. Deletion analysis identified the approximate extent of the nosA region which was sequenced, and it was found to contain an open reading frame encoding 683 amino acids including a presumed signal sequence of 44 amino acids. The putative processed form had a molecular weight of 80,218, characteristic typical of outer membrane proteins, and considerable amino acid sequence homology with Escherichia coli BtuB. A short stretch of amino acids homologous with the E. coli TonB-dependent outer membrane proteins, BtuB, IutA, FepA, and FhuA, suggesting a homologous function: interaction with a periplasmic protein or uptake of a specific substrate.

Authors:
; ;  [1];  [2]
  1. Univ. of California, Davis (United States)
  2. Georgia State Univ., Atlanta (United States)
Publication Date:
OSTI Identifier:
5865628
DOE Contract Number:  
FG03-85ER13356
Resource Type:
Journal Article
Journal Name:
Journal of Bacteriology; (United States)
Additional Journal Information:
Journal Volume: 173:17; Journal ID: ISSN 0021-9193
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; OXIDOREDUCTASES; GENES; PSEUDOMONAS; MOLECULAR BIOLOGY; AMINO ACID SEQUENCE; COPPER; DNA SEQUENCING; DNA-CLONING; ESCHERICHIA COLI; MUTANTS; NITROUS OXIDE; BACTERIA; CHALCOGENIDES; CLONING; DNA HYBRIDIZATION; ELEMENTS; ENZYMES; HYBRIDIZATION; METALS; MICROORGANISMS; MOLECULAR STRUCTURE; NITROGEN COMPOUNDS; NITROGEN OXIDES; ORGANIC COMPOUNDS; OXIDES; OXYGEN COMPOUNDS; PROTEINS; STRUCTURAL CHEMICAL ANALYSIS; TRANSITION ELEMENTS; 550200* - Biochemistry

Citation Formats

Lee, H S, Clark, M A, Ingraham, J L, and Abdelal, A H.T. Molecular characterization of nosA, a Pseudomonas stutzeri gene encoding an outer membrane protein required to make copper-containing N sub 2 O reductase. United States: N. p., 1991. Web.
Lee, H S, Clark, M A, Ingraham, J L, & Abdelal, A H.T. Molecular characterization of nosA, a Pseudomonas stutzeri gene encoding an outer membrane protein required to make copper-containing N sub 2 O reductase. United States.
Lee, H S, Clark, M A, Ingraham, J L, and Abdelal, A H.T. 1991. "Molecular characterization of nosA, a Pseudomonas stutzeri gene encoding an outer membrane protein required to make copper-containing N sub 2 O reductase". United States.
@article{osti_5865628,
title = {Molecular characterization of nosA, a Pseudomonas stutzeri gene encoding an outer membrane protein required to make copper-containing N sub 2 O reductase},
author = {Lee, H S and Clark, M A and Ingraham, J L and Abdelal, A H.T.},
abstractNote = {A Pseudomonas stutzeri gene (nosA) encoding an outer membrane protein was cloned into the broad-host-range vector pRK290 and expressed in a mutant lacking the protein. Deletion analysis identified the approximate extent of the nosA region which was sequenced, and it was found to contain an open reading frame encoding 683 amino acids including a presumed signal sequence of 44 amino acids. The putative processed form had a molecular weight of 80,218, characteristic typical of outer membrane proteins, and considerable amino acid sequence homology with Escherichia coli BtuB. A short stretch of amino acids homologous with the E. coli TonB-dependent outer membrane proteins, BtuB, IutA, FepA, and FhuA, suggesting a homologous function: interaction with a periplasmic protein or uptake of a specific substrate.},
doi = {},
url = {https://www.osti.gov/biblio/5865628}, journal = {Journal of Bacteriology; (United States)},
issn = {0021-9193},
number = ,
volume = 173:17,
place = {United States},
year = {Sun Sep 01 00:00:00 EDT 1991},
month = {Sun Sep 01 00:00:00 EDT 1991}
}