skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Gamma-aminobutyric acid-modulated benzodiazepine binding sites in bacteria

Abstract

Benzodiazepine binding sites, which were once considered to exist only in higher vertebrates, are here demonstrated in the bacteria E. coli. The bacterial ({sup 3}H)diazepam binding sites are modulated by GABA; the modulation is dose dependent and is reduced at high concentrations. The most potent competitors of E.Coli ({sup 3}H)diazepam binding are those that are active in displacing ({sup 3}H)benzodiazepines from vertebrate peripheral benzodiazepine binding sites. These vertebrate sites are not modulated by GABA, in contrast to vertebrate neuronal benzodiazepine binding sites. The E.coli benzodiazepine binding sites therefore differ from both classes of vertebrate benzodiazepine binding sites; however the ligand spectrum and GABA-modulatory properties of the E.coli sites are similar to those found in insects. This intermediate type of receptor in lower species suggests a precursor for at least one class of vertebrate benzodiazepine binding sites may have existed.

Authors:
;  [1];  [2];  [3]
  1. (Univ. of Sydney, New South Wales (Australia))
  2. (Royal Melbourne Inst. of Tech. (Australia))
  3. (CSIRO, Melbourne (Australia))
Publication Date:
OSTI Identifier:
5865500
Resource Type:
Journal Article
Journal Name:
Life Sciences; (United States)
Additional Journal Information:
Journal Volume: 49:15; Journal ID: ISSN 0024-3205
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; AMINOBUTYRIC ACID; BIOCHEMICAL REACTION KINETICS; ESCHERICHIA COLI; BIOCHEMISTRY; SYMPATHOMIMETICS; RECEPTORS; DOSE-RESPONSE RELATIONSHIPS; LIGANDS; TRACER TECHNIQUES; ULTRAVIOLET RADIATION; AMINO ACIDS; AUTONOMIC NERVOUS SYSTEM AGENTS; BACTERIA; CARBOXYLIC ACIDS; CHEMISTRY; DRUGS; ELECTROMAGNETIC RADIATION; ISOTOPE APPLICATIONS; KINETICS; MEMBRANE PROTEINS; MICROORGANISMS; NEUROREGULATORS; ORGANIC ACIDS; ORGANIC COMPOUNDS; PROTEINS; RADIATIONS; REACTION KINETICS; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Lummis, S.C.R., Johnston, G.A.R., Nicoletti, G., and Holan, G. Gamma-aminobutyric acid-modulated benzodiazepine binding sites in bacteria. United States: N. p., 1991. Web. doi:10.1016/0024-3205(91)90595-3.
Lummis, S.C.R., Johnston, G.A.R., Nicoletti, G., & Holan, G. Gamma-aminobutyric acid-modulated benzodiazepine binding sites in bacteria. United States. doi:10.1016/0024-3205(91)90595-3.
Lummis, S.C.R., Johnston, G.A.R., Nicoletti, G., and Holan, G. Tue . "Gamma-aminobutyric acid-modulated benzodiazepine binding sites in bacteria". United States. doi:10.1016/0024-3205(91)90595-3.
@article{osti_5865500,
title = {Gamma-aminobutyric acid-modulated benzodiazepine binding sites in bacteria},
author = {Lummis, S.C.R. and Johnston, G.A.R. and Nicoletti, G. and Holan, G.},
abstractNote = {Benzodiazepine binding sites, which were once considered to exist only in higher vertebrates, are here demonstrated in the bacteria E. coli. The bacterial ({sup 3}H)diazepam binding sites are modulated by GABA; the modulation is dose dependent and is reduced at high concentrations. The most potent competitors of E.Coli ({sup 3}H)diazepam binding are those that are active in displacing ({sup 3}H)benzodiazepines from vertebrate peripheral benzodiazepine binding sites. These vertebrate sites are not modulated by GABA, in contrast to vertebrate neuronal benzodiazepine binding sites. The E.coli benzodiazepine binding sites therefore differ from both classes of vertebrate benzodiazepine binding sites; however the ligand spectrum and GABA-modulatory properties of the E.coli sites are similar to those found in insects. This intermediate type of receptor in lower species suggests a precursor for at least one class of vertebrate benzodiazepine binding sites may have existed.},
doi = {10.1016/0024-3205(91)90595-3},
journal = {Life Sciences; (United States)},
issn = {0024-3205},
number = ,
volume = 49:15,
place = {United States},
year = {1991},
month = {1}
}