Down-regulation of endothelin receptors in the ventrolateral medulla of spontaneously hypertensive rats
- Univ. of Illinois, Chicago (USA)
The binding of ({sup 125}I) sarafotoxin 6b (SRT 6b) and ({sup 125}I) endothelin-1 (ET-1) to endothelin (ET) receptors of neuronal membranes prepared from cerebral cortex and ventrolateral medulla of 8 week old spontaneously hypertensive (SHR) and normotensive Wistar-Kyoto (WKY) rats was determined. ({sup 125}I) SRT 6b bound to the membranes of cerebral cortex and ventrolateral medulla at a single high affinity site. The binding of ({sup 125}I) SRT 6b in the cerebral cortex was found to be similar in SHR and WKY rats. However, in the ventrolateral medulla ({sup 125}I) SRT 6b binding was found to be significantly lower in SHR as compared to WKY rats. The decreased binding was due to decrease (48%) in the B{sub max} values in SHR rats as compared to WKY rats. The K{sub d} values were similar in SHR and WKY rats. ({sup 125}I) ET-1 also bound to the membranes of cerebral cortex and ventrolateral medulla at a single high affinity site. The binding of ({sup 125}I) ET-1 in the cerebral cortex was found to be similar in SHR and WKY rats. However, in the ventrolateral medulla ({sup 125}I) ET-1 binding was found to be significantly lower in SHR as compared to WKY rats. The decreased binding was due to 36% decrease in the B{sub max} values in SHR rats as compared to WKY rats. The K{sub d} values were similar in SHR and WKY rats. It is concluded that the population of ET receptors is less in the ventrolateral medulla of SHR rats and may be contributing to the regulation of blood pressure.
- OSTI ID:
- 5857669
- Journal Information:
- Life Sciences; (USA), Vol. 48:12; ISSN 0024-3205
- Country of Publication:
- United States
- Language:
- English
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