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Title: Solubilization of glycoproteins of envelope viruses by detergents

Abstract

The action of a number of known ionic and nonionic detergents, as well as the new nonionic detergent MESK, on envelope viruses was investigated. It was shown that the nonionic detergents MESK, Triton X-100, and octyl-..beta..-D-glucopyranoside selectively solubilize the outer glycoproteins of the virus particles. The nonionic detergent MESK has the mildest action. Using MESK, purified glycoproteins of influenza, parainfluenza, Venezuelan equine encephalomyelitis, vesicular stomatitis, rabies, and herpes viruses were obtained. The procedure for obtaining glycoproteins includes incubation of the virus suspension with the detergent MESK, removal of subvirus structures by centrifuging, and purification of glycoproteins from detergents by dialysis. Isolated glycoproteins retain a native structure and biological activity and possess high immunogenicity. The detergent MESK is promising for laboratory tests and with respect to the production of subunit vaccines.

Authors:
; ; ; ;
Publication Date:
Research Org.:
D.I. Ivanovskii Institute of Virology, Moscow, USSR
OSTI Identifier:
5856548
Resource Type:
Journal Article
Journal Name:
Biochemistry (Engl. Transl.); (United States)
Additional Journal Information:
Journal Volume: 51:5; Other Information: Translated from Biokhimiya; 51: No. 5, 808-815(May 1986)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; DETERGENTS; BIOLOGICAL EFFECTS; LABELLING; GLUCOPROTEINS; DISSOLUTION; RADIOIMMUNOASSAY; VIRUSES; BIODEGRADATION; CARBON 14 COMPOUNDS; COMPARATIVE EVALUATIONS; ELECTROPHORESIS; HEMAGGLUTININS; IMMUNE REACTIONS; MATERIALS TESTING; RADIOACTIVITY; SOLUBILITY; ADDITIVES; AGGLUTININS; ANTIBODIES; CARBOHYDRATES; CHEMICAL REACTIONS; DECOMPOSITION; EMULSIFIERS; IMMUNOASSAY; IMMUNOLOGY; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MICROORGANISMS; ORGANIC COMPOUNDS; PARASITES; PROTEINS; RADIOASSAY; RADIOIMMUNOLOGY; SACCHARIDES; SURFACTANTS; TESTING; TRACER TECHNIQUES; WETTING AGENTS; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Berezin, V.E., Zaides, V.M., Artamsnov, A.F., Isaeva, E.S., and Zhdanov, V.M. Solubilization of glycoproteins of envelope viruses by detergents. United States: N. p., 1986. Web.
Berezin, V.E., Zaides, V.M., Artamsnov, A.F., Isaeva, E.S., & Zhdanov, V.M. Solubilization of glycoproteins of envelope viruses by detergents. United States.
Berezin, V.E., Zaides, V.M., Artamsnov, A.F., Isaeva, E.S., and Zhdanov, V.M. Thu . "Solubilization of glycoproteins of envelope viruses by detergents". United States.
@article{osti_5856548,
title = {Solubilization of glycoproteins of envelope viruses by detergents},
author = {Berezin, V.E. and Zaides, V.M. and Artamsnov, A.F. and Isaeva, E.S. and Zhdanov, V.M.},
abstractNote = {The action of a number of known ionic and nonionic detergents, as well as the new nonionic detergent MESK, on envelope viruses was investigated. It was shown that the nonionic detergents MESK, Triton X-100, and octyl-..beta..-D-glucopyranoside selectively solubilize the outer glycoproteins of the virus particles. The nonionic detergent MESK has the mildest action. Using MESK, purified glycoproteins of influenza, parainfluenza, Venezuelan equine encephalomyelitis, vesicular stomatitis, rabies, and herpes viruses were obtained. The procedure for obtaining glycoproteins includes incubation of the virus suspension with the detergent MESK, removal of subvirus structures by centrifuging, and purification of glycoproteins from detergents by dialysis. Isolated glycoproteins retain a native structure and biological activity and possess high immunogenicity. The detergent MESK is promising for laboratory tests and with respect to the production of subunit vaccines.},
doi = {},
journal = {Biochemistry (Engl. Transl.); (United States)},
number = ,
volume = 51:5,
place = {United States},
year = {1986},
month = {11}
}