skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Differential expression of RUBISCO small subunit genes during adaptation to limiting CO sub 2 in Chlamydomonas reinhardtii

Abstract

In Chlamydomonas reinhardtii, the small subunit (SS) of RUBISCO is encoded by a two-member gene family which transcribes messages of 1.1 kb (rbcS1 gene) and 0.85 kb (rbcS2 gene). The two genes are regulated differently depending on conditions of growth. For example, while adapting to limiting CO{sub 2} (0.03% CO{sub 2} in air) from a high CO{sub 2} environment (5% CO{sub 2} in air) wild-type C. reinhardtii cells express progressively less 1.1 kb message while levels of the 0.85 kb message remain relatively constant. Between 4 and 8 hours of adaptation, the 1.1 kb message cannot be detected on RNA blots, but by 24 hours it has again accumulated to about the level found in 2-hour adapted cells. In vivo synthesis of RUBISCO SS polypeptide during adaptation to a limiting CO{sub 2} environment parallels expression of the rbcS1 gene and not the rbcS2 gene. It may be that a yet unidentified signal responsible for expression of a group of polypeptides during adaptation to limiting CO{sub 2}, also regulates rbcS1 (but not rbcS2) gene expression.

Authors:
;  [1]
  1. (Iowa State Univ., Ames (USA))
Publication Date:
OSTI Identifier:
5855971
Alternate Identifier(s):
OSTI ID: 5855971
Report Number(s):
CONF-9007196--
Journal ID: ISSN 0079-2241; CODEN: PPYSA
Resource Type:
Conference
Journal Name:
Plant Physiology, Supplement; (USA)
Additional Journal Information:
Journal Volume: 93:1; Conference: Annual meeting of the American Society of Plant Physiologists, Indianapolis, IN (USA), 29 Jul - 2 Aug 1990; Journal ID: ISSN 0079-2241
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; CARBON DIOXIDE; BIOCHEMICAL REACTION KINETICS; CHLAMYDOMONAS; GENES; POLYPEPTIDES; RIBULOSE DIPHOSPHATE CARBOXYLASE; ALGAE; CARBON COMPOUNDS; CARBON OXIDES; CARBON-CARBON LYASES; CARBOXY-LYASES; CHALCOGENIDES; CHLOROPHYCOTA; ENZYMES; KINETICS; LYASES; MICROORGANISMS; ORGANIC COMPOUNDS; OXIDES; OXYGEN COMPOUNDS; PEPTIDES; PLANTS; PROTEINS; REACTION KINETICS; UNICELLULAR ALGAE 550200* -- Biochemistry; 540110

Citation Formats

Winder, T.L., and Spalding, M.H. Differential expression of RUBISCO small subunit genes during adaptation to limiting CO sub 2 in Chlamydomonas reinhardtii. United States: N. p., 1990. Web.
Winder, T.L., & Spalding, M.H. Differential expression of RUBISCO small subunit genes during adaptation to limiting CO sub 2 in Chlamydomonas reinhardtii. United States.
Winder, T.L., and Spalding, M.H. Tue . "Differential expression of RUBISCO small subunit genes during adaptation to limiting CO sub 2 in Chlamydomonas reinhardtii". United States.
@article{osti_5855971,
title = {Differential expression of RUBISCO small subunit genes during adaptation to limiting CO sub 2 in Chlamydomonas reinhardtii},
author = {Winder, T.L. and Spalding, M.H.},
abstractNote = {In Chlamydomonas reinhardtii, the small subunit (SS) of RUBISCO is encoded by a two-member gene family which transcribes messages of 1.1 kb (rbcS1 gene) and 0.85 kb (rbcS2 gene). The two genes are regulated differently depending on conditions of growth. For example, while adapting to limiting CO{sub 2} (0.03% CO{sub 2} in air) from a high CO{sub 2} environment (5% CO{sub 2} in air) wild-type C. reinhardtii cells express progressively less 1.1 kb message while levels of the 0.85 kb message remain relatively constant. Between 4 and 8 hours of adaptation, the 1.1 kb message cannot be detected on RNA blots, but by 24 hours it has again accumulated to about the level found in 2-hour adapted cells. In vivo synthesis of RUBISCO SS polypeptide during adaptation to a limiting CO{sub 2} environment parallels expression of the rbcS1 gene and not the rbcS2 gene. It may be that a yet unidentified signal responsible for expression of a group of polypeptides during adaptation to limiting CO{sub 2}, also regulates rbcS1 (but not rbcS2) gene expression.},
doi = {},
journal = {Plant Physiology, Supplement; (USA)},
issn = {0079-2241},
number = ,
volume = 93:1,
place = {United States},
year = {1990},
month = {5}
}

Conference:
Other availability
Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that hold this conference proceeding.

Save / Share: