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Title: /sup 45/Ca distribution and transport in saponin skinned vascular smooth muscle

Abstract

/sup 45/Ca distribution and transport were studied in chemically skinned strips of caudal artery from Kyoto Wistar rats. Sarcolemmal membranes were made hyperpermeable by exposure for 60 min to solutions containing 0.1 mg/ml of saponin. Skinned helical strips responded with graded contractions to changes in ethylene glycol bis-(beta-aminoethyl ether)-N,N'-tetraacetic acid buffered free Ca solutions (10(-7) to 10(-5) M) and were sensitive to the Mg-ATP concentration. Tissues loaded in the presence of 10(-7) M Ca contracted in response to 10 mM caffeine. These experiments indicate the strips are skinned and possess a functional regulatory and contractile system and an intact Ca sequestering system. /sup 45/Ca distributes in three compartments in skinned caudal artery strips. The Ca contents of two components are linear functions of the Ca-ethylene glycol bis-(beta-aminoethyl ether)-N,N'-tetraacetic acid concentration and desaturate at rapid rates. They correspond to the extracellular and cytoplasmic spaces. A significantly smaller component releases Ca at comparatively slower rates. /sup 45/Ca uptake by the slow component consists of an ATP-dependent and an ATP-independent fraction. The /sup 45/Ca content of the ATP-dependent fraction is a function of the free Ca concentration and is independent of the Ca-ethylene glycol bis-(beta-aminoethyl ether)-N,N'-tetraacetic acid concentration. Its content was enhanced bymore » oxalate and was abolished by Triton X-100 skinning solutions. The ATP-independent component was not affected by Triton X-100 skinning and may represent Ca binding to cytoplasmic molecules and structures. The sequestered Ca was released with caffeine or Ca but not by epinephrine. The observations indicate that the sarcoplasmic reticulum and mitochondria of vascular smooth muscle strips skinned with saponin retain their functional integrity after saponin skinning.« less

Authors:
;
Publication Date:
Research Org.:
Department of Physiology, University of Medicine and Dentistry of New Jersey Medical School, Newark
OSTI Identifier:
5852960
Alternate Identifier(s):
OSTI ID: 5852960
Resource Type:
Journal Article
Resource Relation:
Journal Name: J. Pharmacol. Exp. Ther.; (United States); Journal Volume: 225:1
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ARTERIES; RADIONUCLIDE KINETICS; CALCIUM 45; UPTAKE; ATP; GLYCOLS; MUSCLES; RATS; SAPONINS; ALCOHOLS; ALKALINE EARTH ISOTOPES; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BLOOD VESSELS; BODY; CALCIUM ISOTOPES; CARBOHYDRATES; CARDIOVASCULAR SYSTEM; DAYS LIVING RADIOISOTOPES; EVEN-ODD NUCLEI; GLYCOSIDES; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; ISOTOPES; MAMMALS; NUCLEI; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANS; RADIOISOTOPES; RODENTS; VERTEBRATES 560172* -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Animals-- (-1987)

Citation Formats

Stout, M.A., and Diecke, F.P. /sup 45/Ca distribution and transport in saponin skinned vascular smooth muscle. United States: N. p., 1983. Web.
Stout, M.A., & Diecke, F.P. /sup 45/Ca distribution and transport in saponin skinned vascular smooth muscle. United States.
Stout, M.A., and Diecke, F.P. Fri . "/sup 45/Ca distribution and transport in saponin skinned vascular smooth muscle". United States.
@article{osti_5852960,
title = {/sup 45/Ca distribution and transport in saponin skinned vascular smooth muscle},
author = {Stout, M.A. and Diecke, F.P.},
abstractNote = {/sup 45/Ca distribution and transport were studied in chemically skinned strips of caudal artery from Kyoto Wistar rats. Sarcolemmal membranes were made hyperpermeable by exposure for 60 min to solutions containing 0.1 mg/ml of saponin. Skinned helical strips responded with graded contractions to changes in ethylene glycol bis-(beta-aminoethyl ether)-N,N'-tetraacetic acid buffered free Ca solutions (10(-7) to 10(-5) M) and were sensitive to the Mg-ATP concentration. Tissues loaded in the presence of 10(-7) M Ca contracted in response to 10 mM caffeine. These experiments indicate the strips are skinned and possess a functional regulatory and contractile system and an intact Ca sequestering system. /sup 45/Ca distributes in three compartments in skinned caudal artery strips. The Ca contents of two components are linear functions of the Ca-ethylene glycol bis-(beta-aminoethyl ether)-N,N'-tetraacetic acid concentration and desaturate at rapid rates. They correspond to the extracellular and cytoplasmic spaces. A significantly smaller component releases Ca at comparatively slower rates. /sup 45/Ca uptake by the slow component consists of an ATP-dependent and an ATP-independent fraction. The /sup 45/Ca content of the ATP-dependent fraction is a function of the free Ca concentration and is independent of the Ca-ethylene glycol bis-(beta-aminoethyl ether)-N,N'-tetraacetic acid concentration. Its content was enhanced by oxalate and was abolished by Triton X-100 skinning solutions. The ATP-independent component was not affected by Triton X-100 skinning and may represent Ca binding to cytoplasmic molecules and structures. The sequestered Ca was released with caffeine or Ca but not by epinephrine. The observations indicate that the sarcoplasmic reticulum and mitochondria of vascular smooth muscle strips skinned with saponin retain their functional integrity after saponin skinning.},
doi = {},
journal = {J. Pharmacol. Exp. Ther.; (United States)},
number = ,
volume = 225:1,
place = {United States},
year = {Fri Apr 01 00:00:00 EST 1983},
month = {Fri Apr 01 00:00:00 EST 1983}
}