Measurement of CA1P and CA in leaves

Conference · Tue May 01 00:00:00 EDT 1990 · Plant Physiology, Supplement; (USA)

OSTI ID:5851111

Moore, B d; Kobza, J; Seemann, J R ^[1]

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1. Univ. of Nevada, Reno (USA)

Carboxyarabinitol-1-phosphate (CA1P) and carboxyarabinitol (CA) were assayed in leaves by isotope dilution. {sup 14}C-labeled standards were synthesized from (2-{sup 14}C) CABP using acid (CA1P) or alkaline (CA) phosphatase. Either was added to boiling 80% EtOH along with liquid N{sub 2}-killed leaves. Each was largely purified by anion exchange chromatography. CA1P samples were subjected to 2D-TLE/TLC. The specific activity of the {sup 14}C-containing spot was measured using alkaline phosphatase. CA samples were run on an HPLC and the specific activity was determined using a UV monitor and a flow-through radioisotope detector. In 3 of the tested species, light/dark amount of CA1P (nmol/mg Chl) were kidney bean, 0.7/67; sugar beet, 0.8/33; and Alocasia, 0/3.4. Light/dark CA levels (nmol/mg Chl) in these respective species were 897/653, 3.2/3.5, and 5.7/4.6. These results support the hypothesis that CA is a product of CA1P metabolism in vivo under high light, but also indicate that CA is not the only intermediate involved in CA1P synthesis under low light/dark conditions.

• OSTI ID: 5851111
• Report Number(s): CONF-9007196-; CODEN: PPYSA
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 93:1; Conference: Annual meeting of the American Society of Plant Physiologists, Indianapolis, IN (USA), 29 Jul - 2 Aug 1990; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
54 ENVIRONMENTAL SCIENCES
VISIBLE RADIATION
BIOLOGICAL EFFECTS
ALCOHOLS
CARBON 14 COMPOUNDS
LEAVES
PHOSPHORUS COMPOUNDS
TRACER TECHNIQUES
ELECTROMAGNETIC RADIATION
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
RADIATIONS
550201* - Biochemistry- Tracer Techniques
540110