Modulation of acetone-butanol-ethanol fermentation by carbon monoxide and organic acids
Metabolic modulation of acetone-butanol-ethanol fermentation by Clostridium acetobutylicum with carbon monoxide (CO) and organic acids is described. CO, which is a known inhibitor of hydrogenase, was found to be effective in the concentration range of dissolved CO corresponding to a CO partial pressure of 0.1 to 0.2 atm. Metabolic modulation by CO was particularly effective when organic acids such as acetic and butyric acids were added to the fermentation as electron sinks. The uptake of organic acids was enhanced, and increases in butyric acid uptake by 50 to 200% over control were observed. Hydrogen production could be reduced by 50% and the ratio of solvents could be controlled by CO modulation and organic acid addition. Acetone production could be eliminated if desired. Butanol yield could be increased by 10 to 15%. Total solvent yield could be increased 1 to 3% and the electron efficiency to acetone-butanol-ethanol solvents could be increased from 73 to 78% for controls to 80 to 85% for CO- and organic acid-modulated fermentations. Based on these results, the dynamic nature of electron flow in this fermentation has been elucidated and mechanisms for metabolic control have been hypothesized.
- Research Organization:
- CPC International, Summit-Argo, IL
- OSTI ID:
- 5846212
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 49:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
08 HYDROGEN
ACETONE
BIOSYNTHESIS
BUTANOLS
ETHANOL
CARBON MONOXIDE
CHEMICAL REACTION YIELD
CLOSTRIDIUM ACETOBUTYLICUM
ELECTRONS
FERMENTATION
HYDROGEN PRODUCTION
METABOLISM
ORGANIC ACIDS
ORGANIC SOLVENTS
PARTIAL PRESSURE
ALCOHOLS
BACTERIA
BIOCONVERSION
CARBON COMPOUNDS
CARBON OXIDES
CHALCOGENIDES
CLOSTRIDIUM
ELEMENTARY PARTICLES
FERMIONS
HYDROXY COMPOUNDS
KETONES
LEPTONS
METHANOGENIC BACTERIA
MICROORGANISMS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
SOLVENTS
SYNTHESIS
YIELDS
090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)
080106 - Hydrogen- Production- Biosynthesis & Photochemical Processes