Modifications of und rbcS in und Synechococcus sp. PCC7942 resulted in high CO sub 2 requiring mutants

Conference · Tue May 01 00:00:00 EDT 1990 · Plant Physiology, Supplement; (USA)

OSTI ID:5841377

Schwarz, R; Liehman-Hurwitz, J; Martinez, F; Reinhold, L; Kaplan, A ^[1]

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1. Hebrew Univ. of Jerusalem (Israel)

High CO{sub 2} requiring mutants (HCR), capable of growing in the presence of kanamycin, were obtained following transformation with a construct containing {und nptII} in the coding region of rbcS (encoding for the small subunit (SS) of Rubisco). The apparent photosynthetic affinity for inorganic carbon was very low in mutant EK6 whereas in mutant JR12 it was only slightly lower than in high-CO{sub 2}-grown wild type. Southern analysis indicated double crossing over in the case of EK6. In mutant JR12 Southern analysis revealed some rearrangement and a deletion of a region downstream fro {und rbcS}. Western analysis demonstrated normal SS in JR12 but a larger (by about 3 kD) SS in EK6. Sequence analysis indicated that the latter was the result of fusion between {und rbcS} and the flanking region of {und nptII}.

• OSTI ID: 5841377
• Report Number(s): CONF-9007196-; CODEN: PPYSA
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 93:1; Conference: Annual meeting of the American Society of Plant Physiologists, Indianapolis, IN (USA), 29 Jul - 2 Aug 1990; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ALGAE
MUTANTS
CARBON DIOXIDE
METABOLISM
PHOTOSYNTHESIS
RIBULOSE DIPHOSPHATE CARBOXYLASE
CARBON COMPOUNDS
CARBON OXIDES
CARBON-CARBON LYASES
CARBOXY-LYASES
CHALCOGENIDES
CHEMICAL REACTIONS
ENZYMES
LYASES
OXIDES
OXYGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PLANTS
SYNTHESIS
550500* - Metabolism