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Title: Expression of mouse metallothionein genes in tobacco

Abstract

We have expressed a mouse metallothionein (NT) gene in tobacco under control of the cauliflower mosaic virus (CaMV) 35S promoter and a pea ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) gene promoter. Seedlings in which MT gene expression is driven by the 35S promoter are resistant to toxic levels of cadmium. Mature plants carrying the 35S-MT gene accumulate less Cd in their leaves when exposed to low levels of Cd in laboratory growth conditions. Plants with the rbcS-MT construction express this gene in a light-regulated and tissue-specific manner, as expected. Moreover, the MT levels in leaves in these plants are about 20% of those seen in 35S-MT plants. These plants are currently being tested for Cd resistance. In addition, a small field evaluation of 35S-MT lines for Cd levels is being evaluated. These experiments will address the possibility of using MTs to alter Cd levels in crop species.

Authors:
; ; ;  [1]
  1. (Univ. of Kentucky, Lexington (USA))
Publication Date:
OSTI Identifier:
5839634
Alternate Identifier(s):
OSTI ID: 5839634
Report Number(s):
CONF-9007196--
Journal ID: ISSN 0079-2241; CODEN: PPYSA
Resource Type:
Conference
Journal Name:
Plant Physiology, Supplement; (USA)
Additional Journal Information:
Journal Volume: 93:1; Conference: Annual meeting of the American Society of Plant Physiologists, Indianapolis, IN (USA), 29 Jul - 2 Aug 1990; Journal ID: ISSN 0079-2241
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; CADMIUM; TOXICITY; METALLOTHIONEIN; BIOLOGICAL EFFECTS; NICOTIANA; RIBULOSE DIPHOSPHATE CARBOXYLASE; SULFUR 35; SULFUR COMPOUNDS; TRACER TECHNIQUES; VIRUSES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBON-CARBON LYASES; CARBOXY-LYASES; DAYS LIVING RADIOISOTOPES; ELEMENTS; ENZYMES; EVEN-ODD NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; LYASES; MAGNOLIOPHYTA; MAGNOLIOPSIDA; METALLOPROTEINS; METALS; MICROORGANISMS; NUCLEI; ORGANIC COMPOUNDS; PARASITES; PLANTS; PROTEINS; RADIOISOTOPES; SULFUR ISOTOPES 560300* -- Chemicals Metabolism & Toxicology; 550201 -- Biochemistry-- Tracer Techniques

Citation Formats

Maiti, I.B., Yeargan, R., Wagner, G.J., and Hunt, A.G. Expression of mouse metallothionein genes in tobacco. United States: N. p., 1990. Web.
Maiti, I.B., Yeargan, R., Wagner, G.J., & Hunt, A.G. Expression of mouse metallothionein genes in tobacco. United States.
Maiti, I.B., Yeargan, R., Wagner, G.J., and Hunt, A.G. Tue . "Expression of mouse metallothionein genes in tobacco". United States.
@article{osti_5839634,
title = {Expression of mouse metallothionein genes in tobacco},
author = {Maiti, I.B. and Yeargan, R. and Wagner, G.J. and Hunt, A.G.},
abstractNote = {We have expressed a mouse metallothionein (NT) gene in tobacco under control of the cauliflower mosaic virus (CaMV) 35S promoter and a pea ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) gene promoter. Seedlings in which MT gene expression is driven by the 35S promoter are resistant to toxic levels of cadmium. Mature plants carrying the 35S-MT gene accumulate less Cd in their leaves when exposed to low levels of Cd in laboratory growth conditions. Plants with the rbcS-MT construction express this gene in a light-regulated and tissue-specific manner, as expected. Moreover, the MT levels in leaves in these plants are about 20% of those seen in 35S-MT plants. These plants are currently being tested for Cd resistance. In addition, a small field evaluation of 35S-MT lines for Cd levels is being evaluated. These experiments will address the possibility of using MTs to alter Cd levels in crop species.},
doi = {},
journal = {Plant Physiology, Supplement; (USA)},
issn = {0079-2241},
number = ,
volume = 93:1,
place = {United States},
year = {1990},
month = {5}
}

Conference:
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