Changes in transfer RNA's in human malignant trophoblastic cells (BeWo line)
Ten aminoacyl transfer RNA's prepared from human malignant trophoblastic cells (BeWo line) were compared with the corresponding aminoacyl transfer RNA's from normal human chorionic tissue by cochromatography on a RPC-5 column. Phenylalanyl transfer RNA (Phe-tRNA) of BeWo cells had, in addition to the single species of Phe-tRNA found in normal chorionic tissues, an early eluting component. When Phe-tRNA from the chorion was exposed to mild acid, which selectively excises the Y base, it eluted in the same position as the early eluting Phe-tRNA of BeWo cells. Therefore, the BeWo Phe-tRNA is partially undermodified. Tyrosyl transfer RNA of BeWo cells exhibited a broad-based peak which eluted later than the normal and probably consists of two or more tyrosyl transfer TNA's. Seryl transfer RNA of BeWo cells showed two peaks of acceptor activity, while seryl transfer RNA of normal chorion had a third peak that eluted at a higher salt concentration. In addition, in an early eluting methionyl and lysyl transfer RNA and in a late eluting arginyl transfer RNA from BeWo cells and normal chorion, quantitative alterations were detected. The remaining four transfer RNA's leucyl, aspartyl, valyl, and histidyl, from the two sources did not show any significant differences in elution profiles. These alterations of the chromatographic profile appeared to be due to new or altered species of transfer RNA. They were not due to differences in the aminoacyl transfer RNA synthetase. The transfer RNA methyltransferase capacity of the enzymes from BeWo cells was 2-fold higher than that of the enzymes extracted from the chorion.
- Research Organization:
- Univ. of Colorodo, Denver
- OSTI ID:
- 5834847
- Journal Information:
- Cancer Res.; (United States), Vol. 36
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
TRANSFER RNA
CHEMICAL COMPOSITION
CHROMATOGRAPHY
TUMOR CELLS
AMINO ACIDS
ARGININE
BIOCHEMICAL REACTION KINETICS
CELL CULTURES
LIGASES
LYSINE
METHIONINE
PHENYLALANINE
QUANTITATIVE CHEMICAL ANALYSIS
SERINE
TRANSFERASES
TYROSINE
ANIMAL CELLS
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
ENZYMES
HYDROXY ACIDS
KINETICS
LIPOTROPIC FACTORS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
REACTION KINETICS
RNA
SEPARATION PROCESSES
550200* - Biochemistry
550300 - Cytology