Protein-DNA interactions in vivo of an erythroid-specific, human. beta. -globin locus enhancer

Journal Article · Tue Oct 01 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.19.8676· OSTI ID:5822862

Sekhar Reddy, P M; Shen, C K.J. ^[1]

---

1. Univ. of California, Davis (United States)

The 5{prime} DNase I-hypersensitive site 2 (5{prime} HS-2) is an erythroid-specific enhancer located 11 kilobases (kb) upstream of the human {beta}-globin gene cluster. Presence in cis of 5{prime} HS-2 confers a high level of erythroid cell-specific and developmentally regulated promoter activities of human globin genes in transfected cell cultures and in transgenic mice. Combining the use of the methylation protection assay and polymerase chain reaction, the authors have studied nuclear factor-DNA interactions of the 5{prime} HS-2 represent three different modes of protein-DNA interaction with respect to cell-type specificities and in vivo vs. in vitro differences. These data provide direct evidence that specific nuclear factor-DNA complexes form in vivo at functionally important sequence motifs of the 5{prime} HS-2 enhancer in erythroid cells. The detection of conformationally different nuclear-factor-DNA complexes at the same sequence motifs in HeLa and Raji cell lines also raises interesting questions regarding the origin and function of these complexes in nonerythroid cells.

• OSTI ID: 5822862
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:19; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA
CROSS-LINKING
GLOBINS
GENETIC MAPPING
PROTEINS
RECOMBINANT DNA
DNA SEQUENCING
DNA-ASE
ERYTHROCYTES
GENE REPRESSORS
GENES
HELA CELLS
METHYLATION
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL REACTIONS
ENZYMES
ESTERASES
HYDROLASES
MAPPING
MATERIALS
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
POLYMERIZATION
STRUCTURAL CHEMICAL ANALYSIS
550200* - Biochemistry