Cloning of a human cDNA encoding a CDC2-related kinase by complementation of a budding yeast cdc28 mutation

Journal Article · Tue Oct 15 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.20.9006· OSTI ID:5822809

Ninomiya-Tsuji, Jun ^[1]; Nomoto, Satoshi; Matsumoto, Kunihiro ^[2]; Yasuda, Hideyo ^[3]; Reed, S I ^[4]

---

1. DNAX Research Inst., Palo Alto, CA (United States)
2. Nagoya Univ. (Japan)
3. Kanazawa Univ. (Japan)
4. Scripps Research Inst., La Jolla, CA (United States)

The authors have cloned two different human cDNAs that can complement cdc28 mutations of budding yeast Saccharomyces cerevisiae. One corresponds to a gene encoding human p34{sup CDC2} kinase, and the other to a gene (CDK2; cell division kinase) that has not been characterized previously. The CDK2 protein is highly homologous to p34{sup CDC2} kinase and more significantly is homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The human CDC2 and CDK2 genes were both able to complement the inviability of a null allele of S. cerevisiae CDC28. This result indicates that the CDK2 protein has a biological activity closely related to the CDC28 and p34{sup CDC2} kinases. However, CDK2 was unable to complement cdc2 mutants in fission yeast Schizosaccharomyces pombe under the condition where the human CDC2 gene could complement them. CDK2 mRNA appeared late in G{sub 1} or in early S phase, slightly before CDC2 mRNA, after growth stimulation in normal human fibroblast cells. These results suggest that in human cells, two different CDC2-like kinases may regulate the cell cycle at distinct stages.

• OSTI ID: 5822809
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:20; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL CYCLE
GENE REGULATION
RECOMBINANT DNA
DNA SEQUENCING
DNA-CLONING
MAN
PHOSPHOTRANSFERASES
PLASMIDS
SACCHAROMYCES CEREVISIAE
ANIMALS
CELL CONSTITUENTS
CLONING
DNA
DNA HYBRIDIZATION
ENZYMES
EUMYCOTA
FUNGI
HYBRIDIZATION
MAMMALS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
PRIMATES
PROTEINS
SACCHAROMYCES
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VERTEBRATES
YEASTS
550200* - Biochemistry