Cloning and expression of a human kidney cDNA for an /alpha//sub 2/-adrenergic receptor subtype

Journal Article · Thu Sep 01 00:00:00 EDT 1988 · Proc. Natl. Acad. Sci. USA; (United States)

DOI:https://doi.org/10.1073/pnas.85.17.6301· OSTI ID:5822662

Regan, J W; Kobilka, T S; Yang-Feng, T L; Caron, M G; Lefkowitz, R J; Kobilka, B K

An /alpha//sub 2/-adrenergic receptor subtype has been cloned from a human kidney cDNA library using the gene for the human platelet /alpha//sub 2/-adrenergic receptor as a probe. The deduced amino acid sequence resembles the human platelet /alpha//sub 2/-adrenergic receptor and is consistent with the structure of other members of he family of guanine nucleotide-binding protein-coupled receptors. The cDNA was expressed in a mammalian cell line (COS-7), and the /alpha//sub 2/-adrenergic ligand (/sup 3/H)rauwolscine was bound. Competition curve analysis with a variety of adrenergic ligands suggests that this cDNA clone represents the /alpha//sub 2/B-adrenergic receptor. The gene for this receptor is on human chromosome 4, whereas the gene for the human platelet /alpha//sub 2/-adrenergic receptor (/alpha//sub 2/A) lies on chromosome 10. This ability to express the receptor in mammalian cells, free of other adrenergic receptor subtypes, should help in developing more selective /alpha/-adrenergic ligands.

• Research Organization: Howard Hughes Medical Institute, Durham, NC (USA)
• OSTI ID: 5822662
• Journal Information: Proc. Natl. Acad. Sci. USA; (United States), Vol. 85:17
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HETEROCHROMOSOMES
GENETIC MAPPING
RECEPTORS
AMINO ACID SEQUENCE
RECOMBINANT DNA
DNA-CLONING
BIOLOGICAL MODELS
BLOOD PLATELETS
CELL MEMBRANES
GENES
KIDNEYS
LIGANDS
MAN
TRITIUM COMPOUNDS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CELL CONSTITUENTS
CHROMOSOMES
CLONING
DNA
DNA HYBRIDIZATION
HYBRIDIZATION
LABELLED COMPOUNDS
MAMMALS
MAPPING
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques