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Title: (X-ray microimaging by diffractive techniques)

Abstract

Progress is reported on high resolution x-ray techniques to image wet biological specimens. The minimization of radiation damage to the specimens and holographic imaging are described. (CBS)

Publication Date:
Research Org.:
State Univ. of New York, Stony Brook, NY (United States). Research Foundation
Sponsoring Org.:
USDOE; USDOE, Washington, DC (United States)
OSTI Identifier:
5819424
Report Number(s):
DOE/ER/60858-2
ON: DE92005530
DOE Contract Number:
FG02-89ER60858
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; HOLOGRAPHY; DIAGNOSTIC USES; SCANNING LIGHT MICROSCOPY; X RADIATION; BIOLOGICAL RADIATION EFFECTS; CHROMOSOMES; FOURIER TRANSFORMATION; IMAGES; PROGRESS REPORT; RESOLUTION; BIOLOGICAL EFFECTS; DOCUMENT TYPES; ELECTROMAGNETIC RADIATION; INTEGRAL TRANSFORMATIONS; IONIZING RADIATIONS; MICROSCOPY; RADIATION EFFECTS; RADIATIONS; TRANSFORMATIONS; USES; 550602* - Medicine- External Radiation in Diagnostics- (1980-)

Citation Formats

Not Available. (X-ray microimaging by diffractive techniques). United States: N. p., 1991. Web. doi:10.2172/5819424.
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Not Available. (X-ray microimaging by diffractive techniques). United States. doi:10.2172/5819424.
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Not Available. Tue . "(X-ray microimaging by diffractive techniques)". United States. doi:10.2172/5819424. https://www.osti.gov/servlets/purl/5819424.
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@article{osti_5819424,
title = {(X-ray microimaging by diffractive techniques)},
author = {Not Available},
abstractNote = {Progress is reported on high resolution x-ray techniques to image wet biological specimens. The minimization of radiation damage to the specimens and holographic imaging are described. (CBS)},
doi = {10.2172/5819424},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 1991},
month = {Tue Jan 01 00:00:00 EST 1991}
}
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Technical Report:
View Technical Report (2.59 MB)
DOI:  10.2172/5819424
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  • The first year of the research program resulted in significant progress on several fronts. We had a rapid start, because the soft x-ray undulator beamline, X1A, at the National Synchrotron Light Source became operational shortly before the start of the grant period. In microimaging by diffraction, Dr. David Sayre was successful in recording moderately large angle diffraction patterns from single diatom specimens. The patterns (Fig. 1) correspond to information down to the 70 Angstrom level, and are by far the best achieved to date. To obtain these, a new diffraction camera was constructed and commissioned. It features a multiple pinholemore » collimator to define a 10 micron diameter beam, and an alignment system to position the 2 micron specimen within it. 2 figs.« less

  • Progress is reported on high resolution x-ray techniques to image wet biological specimens. The minimization of radiation damage to the specimens and holographic imaging are described. (CBS)

  • ;
    The report summarizes the development of soft x-ray microscopes at the National Synchrotron Light Source X-1A beamline. We have developed a soft x-ray microscopy beamline (X-1A) at the National Synchrotron Light Source at Brookhaven National Laboratory. This beamline has been upgraded recently to provide two endstations dedicated to microscopy experiments. One endstation hosts a brand new copy of the redesigned room temperature scanning x-ray microscope (STXM), and the other end station hosts a cryo STXM and the original redesigned room temperature microscope, which has been commissioned and has started operation. Cryo STXM and the new microscope use the same newmore » software package, running under the LINUX operating system. The new microscope is showing improved image resolution and extends spectromicroscopy to the nitrogen, oxygen and iron edges. These microscopes are used by us, and by users of the facility, to image hydrated specimens at 50 nm or better spatial resolution and with 0.1-0.5 eV energy resolution. This allows us to carry out chemical state mapping in biological, materials science, and environmental and colloidal science specimens. In the cryo microscope, we are able to do chemical state mapping and tomography of frozen hydrated specimens, and this is of special importance for radiation-sensitive biological specimens. for spectromicroscopic analysis, and methods for obtaining real-space images from the soft x-ray diffraction patterns of non-crystalline specimens. The user program provides opportunities for collaborators and other groups to exploit the techniques available and to develop them further. We have also developed new techniques such as an automated method for acquiring ''stacks'' of images.« less

  • ; ; ; ...
    Phase zone plates of high focusing efficiency and submicron resolution have been demonstrated in the hard x-ray region. A scanning microscope based on these focusing optics will create many new applications. Preliminary results in the applications of the microscope are reported here. In the area of imaging, we have utilized absorption contrast to clearly identify the locations of Au and Ni constituents in a sample of two interleaved grids. Micro-EXAFS spectra has also been obtained on a Ni foil. Fluorescence from a nuclear fuel sample, as an example of microanalysis, has revealed the elemental distribution at the interfaces. Lastly, microdiffractionmore » from AgBr crystallites has been studied. 5 figs, 7 refs.« less

  • ; ; ; ...
    Phase zone plates of high focusing efficiency and submicron resolution have been demonstrated in the hard x-ray region. A scanning microscope based on these focusing optics will create many new applications. Preliminary results in the applications of the microscope are reported here. In the area of imaging, we have utilized absorption contrast to clearly identify the locations of Au and Ni constituents in a sample of two interleaved grids. Micro-EXAFS spectra has also been obtained on a Ni foil. Fluorescence from a nuclear fuel sample, as an example of microanalysis, has revealed the elemental distribution at the interfaces. Lastly, microdiffractionmore » from AgBr crystallites has been studied. 5 figs, 7 refs.« less