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Title: Sensitivity of early mouse embryos to (/sup 3/H)thymidine

Abstract

Effects of intranuclear radiation on the developmental capacity of early mouse embryos were studied by exposing embryos to (/sup 3/H)thymidine and counting the number of embryos forming blastocysts, trophoblast outgrowths, inner cell masses (ICMs), and two-layer ICMs (differentiated into primary endoderm and ectoderm). When embryos were cultured from the 2-cell stage for 8 days in the continuous presence of (/sup 3/H)thymidine, concentrations as low as 0.2 nCi/ml reduced the number of embryos forming two-layer ICMs. At 1 nCi/ml, the number of both ICMs and two-layer ICMs were reduced, and at 10 nCi/ml the number of embryos developing to all three post-blastocyst endpoints was reduced. Blastocyst formation was not affected even at the highst concentration (/sup 3/H)thymidine and then cultured further in unlabelled medium, the effects were similar to those of 8-day exposure. When embryos were exposed to (/sup 3/H)thymidine for 24 h at various developmental stages, effects were less severe than when they were exposed continuously for 3 or 8 days, and the sensitivity of embryos differed between stages. The 24-h exposure of immunosurgically isolated ICMS to (/sup 3/H)thymidine revealed that the high sensitivity of the ICM to (/sup 3/H)thymidine persists through the late blastocyst stage and declines progressively thereafter.more » Autoradiography indicated that the change in radiosensitivity of embryos or ICMs is generally related to their ability to incorporate (/sup 3/H)thymidine into the DNA.« less

Authors:
 [1]; ;
  1. (Univ. of California, San Francisco)
Publication Date:
OSTI Identifier:
5815492
Resource Type:
Journal Article
Journal Name:
Exp. Cell Res.; (United States)
Additional Journal Information:
Journal Volume: 142:2
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; EMBRYOS; INTERNAL IRRADIATION; RADIOSENSITIVITY; AUTORADIOGRAPHY; BIOLOGICAL RADIATION EFFECTS; CELL CULTURES; MICE; THYMIDINE; TRITIUM COMPOUNDS; ANIMALS; AZINES; BIOLOGICAL EFFECTS; HETEROCYCLIC COMPOUNDS; IRRADIATION; LABELLED COMPOUNDS; MAMMALS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIMIDINES; RADIATION EFFECTS; RIBOSIDES; RODENTS; VERTEBRATES; 560162* - Radionuclide Effects, Kinetics, & Toxicology- Animals, Plants, Microorganisms, & Cells

Citation Formats

Spindle, A., Wu, K., and Pedersen, R.A. Sensitivity of early mouse embryos to (/sup 3/H)thymidine. United States: N. p., 1982. Web. doi:10.1016/0014-4827(82)90381-0.
Spindle, A., Wu, K., & Pedersen, R.A. Sensitivity of early mouse embryos to (/sup 3/H)thymidine. United States. doi:10.1016/0014-4827(82)90381-0.
Spindle, A., Wu, K., and Pedersen, R.A. Wed . "Sensitivity of early mouse embryos to (/sup 3/H)thymidine". United States. doi:10.1016/0014-4827(82)90381-0.
@article{osti_5815492,
title = {Sensitivity of early mouse embryos to (/sup 3/H)thymidine},
author = {Spindle, A. and Wu, K. and Pedersen, R.A.},
abstractNote = {Effects of intranuclear radiation on the developmental capacity of early mouse embryos were studied by exposing embryos to (/sup 3/H)thymidine and counting the number of embryos forming blastocysts, trophoblast outgrowths, inner cell masses (ICMs), and two-layer ICMs (differentiated into primary endoderm and ectoderm). When embryos were cultured from the 2-cell stage for 8 days in the continuous presence of (/sup 3/H)thymidine, concentrations as low as 0.2 nCi/ml reduced the number of embryos forming two-layer ICMs. At 1 nCi/ml, the number of both ICMs and two-layer ICMs were reduced, and at 10 nCi/ml the number of embryos developing to all three post-blastocyst endpoints was reduced. Blastocyst formation was not affected even at the highst concentration (/sup 3/H)thymidine and then cultured further in unlabelled medium, the effects were similar to those of 8-day exposure. When embryos were exposed to (/sup 3/H)thymidine for 24 h at various developmental stages, effects were less severe than when they were exposed continuously for 3 or 8 days, and the sensitivity of embryos differed between stages. The 24-h exposure of immunosurgically isolated ICMS to (/sup 3/H)thymidine revealed that the high sensitivity of the ICM to (/sup 3/H)thymidine persists through the late blastocyst stage and declines progressively thereafter. Autoradiography indicated that the change in radiosensitivity of embryos or ICMs is generally related to their ability to incorporate (/sup 3/H)thymidine into the DNA.},
doi = {10.1016/0014-4827(82)90381-0},
journal = {Exp. Cell Res.; (United States)},
number = ,
volume = 142:2,
place = {United States},
year = {1982},
month = {12}
}