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Title: Reliability of a new technique for the determination of vitamin B12 absorption in children: single stool sample test--a double isotope technique

Abstract

The fractional vitamin B12 absorption (FAB12) was determined in 39 patients with various gastrointestinal diseases by a double-isotope technique, employing a single stool sample test (SSST), as well as a complete stool collection. The age of the patients ranged from 2.5 months to 16.2 years (mean 5.0 years). The test dose was administered orally and consisted of 0.5-4.5 micrograms of /sup 57/CoB12 (approximately 0.05 microCi), carmine powder, and 2 mg /sup 51/CrCl/sub 3/ (approximately 1.25 microCi) as the inabsorbable tracer. The wholebody radiation to a 1-year-old child averaged only 20 mrad. The stool and napkin was collected and homogenized by addition of 300 ml chromium sulfuric acid. A 300-ml sample of the homogenized stool and napkin, as well as 300 ml chromium sulfuric acid (75% v/v) containing the standards, were counted in a broad-based well counter. The FAB12 determined by SSST employing the stool with the highest content of /sup 51/Cr (which corresponded to the most carmine-colored stool) correlated closely to the FAB12 based on complete stool collection (r = 0.98, n = 39, p less than 0.001). The reproducibility of FAB12 determined by SSST was assessed from double assays in 19 patients. For a mean value of 12%, themore » SD was 3%, which corresponded to a coefficient of variation (CV) of 25%. The excretion of /sup 57/Co and /sup 51/Cr in the urine was examined in six patients with moderate to severe mucosal damage and was found to be low.« less

Authors:
Publication Date:
Research Org.:
Univ. of Copenhagen, Hellerup, Denmark
OSTI Identifier:
5810436
Resource Type:
Journal Article
Resource Relation:
Journal Name: J. Pediatr. Gastroenterol. Nutr.; (United States); Journal Volume: 2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; FECES; DUAL-ISOTOPE SUBTRACTION TECHNIQUE; VITAMIN B-12; INTESTINAL ABSORPTION; CHILDREN; CHROMIUM 51; COBALT 57; DIGESTIVE SYSTEM DISEASES; EXCRETION; PATIENTS; RADIATION DOSES; RELIABILITY; URINE; ABSORPTION; AGE GROUPS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; BODY FLUIDS; CHROMIUM ISOTOPES; CLEARANCE; COBALT ISOTOPES; DAYS LIVING RADIOISOTOPES; DISEASES; DOSES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; HEMATINICS; HEMATOLOGIC AGENTS; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; RADIOISOTOPES; TRACER TECHNIQUES; UPTAKE; VITAMIN B GROUP; VITAMINS; WASTES; 550501* - Metabolism- Tracer Techniques

Citation Formats

Hjelt, K. Reliability of a new technique for the determination of vitamin B12 absorption in children: single stool sample test--a double isotope technique. United States: N. p., 1986. Web. doi:10.1097/00005176-198605020-00018.
Hjelt, K. Reliability of a new technique for the determination of vitamin B12 absorption in children: single stool sample test--a double isotope technique. United States. doi:10.1097/00005176-198605020-00018.
Hjelt, K. Sat . "Reliability of a new technique for the determination of vitamin B12 absorption in children: single stool sample test--a double isotope technique". United States. doi:10.1097/00005176-198605020-00018.
@article{osti_5810436,
title = {Reliability of a new technique for the determination of vitamin B12 absorption in children: single stool sample test--a double isotope technique},
author = {Hjelt, K.},
abstractNote = {The fractional vitamin B12 absorption (FAB12) was determined in 39 patients with various gastrointestinal diseases by a double-isotope technique, employing a single stool sample test (SSST), as well as a complete stool collection. The age of the patients ranged from 2.5 months to 16.2 years (mean 5.0 years). The test dose was administered orally and consisted of 0.5-4.5 micrograms of /sup 57/CoB12 (approximately 0.05 microCi), carmine powder, and 2 mg /sup 51/CrCl/sub 3/ (approximately 1.25 microCi) as the inabsorbable tracer. The wholebody radiation to a 1-year-old child averaged only 20 mrad. The stool and napkin was collected and homogenized by addition of 300 ml chromium sulfuric acid. A 300-ml sample of the homogenized stool and napkin, as well as 300 ml chromium sulfuric acid (75% v/v) containing the standards, were counted in a broad-based well counter. The FAB12 determined by SSST employing the stool with the highest content of /sup 51/Cr (which corresponded to the most carmine-colored stool) correlated closely to the FAB12 based on complete stool collection (r = 0.98, n = 39, p less than 0.001). The reproducibility of FAB12 determined by SSST was assessed from double assays in 19 patients. For a mean value of 12%, the SD was 3%, which corresponded to a coefficient of variation (CV) of 25%. The excretion of /sup 57/Co and /sup 51/Cr in the urine was examined in six patients with moderate to severe mucosal damage and was found to be low.},
doi = {10.1097/00005176-198605020-00018},
journal = {J. Pediatr. Gastroenterol. Nutr.; (United States)},
number = ,
volume = 2,
place = {United States},
year = {Sat Mar 01 00:00:00 EST 1986},
month = {Sat Mar 01 00:00:00 EST 1986}
}