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Title: Catecholamine transport in cultured bovine adrenal medullary cells: kinetics and mechanism

Abstract

In primary cultures of bovine adrenomedullary cells, catecholamine uptake was found to be a saturable process exhibiting Michaelis-Menten kinetics with an apparent K/sub m/ for 1-norepinephrine of 0.5 ..mu..M. Radiolabeled catecholamines were employed to study the general characteristics and kinetic properties of catecholamine transport in cultured adrenomedullary cells. This process was found to be temperature, energy and Na/sup +/-dependent. In addition, uptake required the presence of extracellular Cl/sup -/, K/sup +/, and divalent cation such as Mn/sup 2 +/, Ca/sup 2 +/, or Mg/sup 2 +/. Agents that induce Ca/sup 2 +/-dependent, exocytotic secretion of catecholamines from these cells had significant inhibitory effects on catecholamine uptake. The secretagogues, nicotine, veratridine and elevated extracellular K/sup +/ concentrations, were all found to inhibit norepinephrine uptake. The inhibitory effects of the secretagogues could be fully demonstrated in the absence of catecholamine secretion. Investigation into the mechanism of catecholamine transport was pursued by measuring the effects of various catecholamine altering conditions or agents on the cellular membrane potential and/or the inwardly directed Na/sup +/ concentration gradient. Changes in the membrane potential were determined biochemically using tetraphenylphosphonium ion distribution, whereas changes in the Na/sup +/-gradient were assessed using /sup 22/Na/sup +/ distribution.

Authors:
Publication Date:
Research Org.:
Duke Univ., Durham, NC (USA)
OSTI Identifier:
5806422
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D.)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CATECHOLAMINES; MEMBRANE TRANSPORT; ADRENAL GLANDS; ANIONS; CATIONS; CATTLE; CELL CULTURES; CELL MEMBRANES; CHLORINE COMPOUNDS; MANGANESE COMPOUNDS; POTASSIUM COMPOUNDS; RESPONSE MODIFYING FACTORS; SODIUM 22; SODIUM COMPOUNDS; TEMPERATURE DEPENDENCE; TRACER TECHNIQUES; ALKALI METAL COMPOUNDS; ALKALI METAL ISOTOPES; AMINES; ANIMALS; AROMATICS; BETA DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; BODY; CELL CONSTITUENTS; CHARGED PARTICLES; DOMESTIC ANIMALS; ENDOCRINE GLANDS; GLANDS; HALOGEN COMPOUNDS; HYDROXY COMPOUNDS; IONS; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MAMMALS; MEMBRANES; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PHENOLS; POLYPHENOLS; RADIOISOTOPES; RUMINANTS; SODIUM ISOTOPES; TRANSITION ELEMENT COMPOUNDS; VERTEBRATES; YEARS LIVING RADIOISOTOPES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Allen, P L. Catecholamine transport in cultured bovine adrenal medullary cells: kinetics and mechanism. United States: N. p., 1985. Web.
Allen, P L. Catecholamine transport in cultured bovine adrenal medullary cells: kinetics and mechanism. United States.
Allen, P L. Tue . "Catecholamine transport in cultured bovine adrenal medullary cells: kinetics and mechanism". United States.
@article{osti_5806422,
title = {Catecholamine transport in cultured bovine adrenal medullary cells: kinetics and mechanism},
author = {Allen, P L},
abstractNote = {In primary cultures of bovine adrenomedullary cells, catecholamine uptake was found to be a saturable process exhibiting Michaelis-Menten kinetics with an apparent K/sub m/ for 1-norepinephrine of 0.5 ..mu..M. Radiolabeled catecholamines were employed to study the general characteristics and kinetic properties of catecholamine transport in cultured adrenomedullary cells. This process was found to be temperature, energy and Na/sup +/-dependent. In addition, uptake required the presence of extracellular Cl/sup -/, K/sup +/, and divalent cation such as Mn/sup 2 +/, Ca/sup 2 +/, or Mg/sup 2 +/. Agents that induce Ca/sup 2 +/-dependent, exocytotic secretion of catecholamines from these cells had significant inhibitory effects on catecholamine uptake. The secretagogues, nicotine, veratridine and elevated extracellular K/sup +/ concentrations, were all found to inhibit norepinephrine uptake. The inhibitory effects of the secretagogues could be fully demonstrated in the absence of catecholamine secretion. Investigation into the mechanism of catecholamine transport was pursued by measuring the effects of various catecholamine altering conditions or agents on the cellular membrane potential and/or the inwardly directed Na/sup +/ concentration gradient. Changes in the membrane potential were determined biochemically using tetraphenylphosphonium ion distribution, whereas changes in the Na/sup +/-gradient were assessed using /sup 22/Na/sup +/ distribution.},
doi = {},
url = {https://www.osti.gov/biblio/5806422}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1985},
month = {1}
}

Thesis/Dissertation:
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