A simplified radioactive assay for the enzyme UDP-glucose pyrophosphorylase

Journal Article · Sun Nov 15 00:00:00 EST 1981 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(81)90164-0· OSTI ID:5801924

Evers, C A; Palatnik, M

A radioactive assay for the enzyme UDP-glucose pyrophosphorylase has been modified. The modifications allow the enzymes to be assayed in one simple step and eliminate two procedures (a boiling step and an alkaline phosphatase incubation) which lengthen the time required to do the assay, make it somewhat cumbersome, and which must be carefully controlled. Furthermore, the modifications allow the simultaneous measurement of large numbers of samples. The modified assay is sensitive enough to quantitate activity in a mutant of Dictyostelium discoideum which with previous spectrophotometric assays could not be accurately measured.

• Research Organization: State Univ. of New York at Stony Brook
• OSTI ID: 5801924
• Journal Information: Anal. Biochem.; (United States), Vol. 118:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ENZYME ACTIVITY
QUANTITATIVE CHEMICAL ANALYSIS
PHOSPHOTRANSFERASES
RADIOASSAY
ISOMERIC NUCLEI
MEASURING METHODS
MUTANTS
MYXOMYCETES
SENSITIVITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE
CHEMICAL ANALYSIS
ENZYMES
FUNGI
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NUCLEI
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
TRANSFERASES
550201* - Biochemistry- Tracer Techniques
550701 - Microbiology- Tracer Techniques