Murine acute leukemia cell line with megakaryocytic differentiation (MK-8057) induced by whole-body irradiation in C sup 3 H/He mice: Cytological properties and kinetics of its leukemic stem cells

Journal Article · Tue Jan 01 00:00:00 EST 1991 · International Journal of Cell Cloning; (USA)

OSTI ID:5799958

Hirabayashi, Y; Inoue, T; Yoshida, K; Sasaki, H; Kubo, S; Kanisawa, M; Seki, M ^[1]

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1. Yokohama City Univ. School of Medicine (Japan)

Five cases of murine leukemia with megakaryocytic differentiation were observed among the 417 cases of radiation-induced leukemias which developed in 30% of C{sup 3}H/HeMs mice exposed at 8 to 10 weeks to 0.5 to 5 gy total body irradiation. Cells from individual leukemic colonies in the spleen of the irradiated mice, and cells from colonies in methylcellulose (MC) culture in vitro, derived from one of these leukemias, MK-8057, were injected into mice; both types of cells caused the deaths of the recipient mice by inducing the same type of leukemia. MK-8057 can be maintained in Dexter-type liquid culture with a feeder layer of irradiated bone marrow cells. There was a linear reciprocal relationship between the increasing number of MK-8057 cells injected versus the survival of the recipient mice. A reciprocal relationship also was seen between an increasing number of leukemic stem cells, corresponding to the number of MK-8057 cells, and the survival of mice injected with MK-8057. Giant nuclear megakaryocytes developed during the course of colony growth in the spleen as they did in the MC culture. Such megakaryocytes were acetylcholinesterase positive, whereas leukemic cells in the peripheral blood showed no sign of platelet production nor of a positive reaction to acetylcholinesterase. Cells maintained in culture were entirely positive in platelet glycoprotein IIb/IIIa when anti-human antibody was used. The larger cells in a splenic cell suspension derived from a moribund mouse were separated and enriched by velocity sedimentation using centrifugal elutriation (CE), and then subjected to flow cytometry using propidium iodide staining. Cells with up to 32N-DNA content were detected. After separating MK-8057 by counter-flow CE, the larger cell fraction produced more leukemic colonies when injected into irradiated mice than did the small cell fraction.

• OSTI ID: 5799958
• Journal Information: International Journal of Cell Cloning; (USA), Vol. 9:1; ISSN 0737-1454
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BONE MARROW CELLS
CELL DIFFERENTIATION
LEUKEMIA
RADIOINDUCTION
BIOLOGICAL RADIATION EFFECTS
CELL CYCLE
CHOLINESTERASE
EXPERIMENTAL NEOPLASMS
MICE
SPLEEN
STEM CELLS
TUMOR CELLS
WHOLE-BODY IRRADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBOXYLESTERASES
CONNECTIVE TISSUE CELLS
DISEASES
ENZYMES
ESTERASES
EXTERNAL IRRADIATION
HEMIC DISEASES
HYDROLASES
IMMUNE SYSTEM DISEASES
IRRADIATION
MAMMALS
NEOPLASMS
ORGANS
RADIATION EFFECTS
RODENTS
SOMATIC CELLS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals