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Title: Acetone and butanol production by Clostridium acetobutylicum in a synthetic medium

Abstract

The effect of the component concentrations of a synthetic medium on acetone and butanol fermentation by Clostridium acetobutylicum ATCC 824 was investigated. Cell growth was dependent on the presence of Mg, Fe, and K in the medium. Mg and Mn had deleterious effects when in excess. Ammonium acetate in excess caused acid fermentation. The metabolism was composed of two phases: an acid phase and a solvent one. Low concentrations of glucose allowed the first phase only. The theoretical ratio of the conversion of glucose to solvents, which was 28 to 33%, was obtained with the following medium: MgSO/sub 4/, 50 to 200 mg/liter; MnSO/sub 4/, 0 to 20 mg/liter; KCl, 0.015 to 8 g/liter (an equivalent concentration of K+ was supplied in the form of KH/sub 2/PO/sub 4/ and K/sub 2/HPO/sub 4/); FeSO/sub 4/, 1 to 50 mg/liter; ammonium acetate, 1.1 to 2.2 g/liter; para-aminobenzoic acid, 1 mg/liter; biotin, 0.01 mg/liter; glucose, 20 to 60 g/liter. (Refs. 24).

Authors:
; ; ;
Publication Date:
Research Org.:
Lab des Sciences du Genie Chimique, CNRS-ENSIC, 54042 Nancy, Cedex, France; Univ de Nancy I, Lab de Chimie Biologique I, 54037 Nancy, Cedex, France
OSTI Identifier:
5792720
Resource Type:
Journal Article
Journal Name:
Appl. Environ. Microbiol.; (United States)
Additional Journal Information:
Journal Volume: 44:6
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; ACETONE; BIOSYNTHESIS; PRODUCTION; BUTANOLS; BIOCHEMICAL REACTION KINETICS; CLOSTRIDIUM ACETOBUTYLICUM; CULTURE MEDIA; GROWTH; HYDROLYSIS; NUTRIENTS; QUANTITY RATIO; ALCOHOLS; BACTERIA; CHEMICAL REACTIONS; CLOSTRIDIUM; DECOMPOSITION; HYDROXY COMPOUNDS; KETONES; KINETICS; LYSIS; METHANOGENIC BACTERIA; MICROORGANISMS; ORGANIC COMPOUNDS; REACTION KINETICS; SOLVOLYSIS; SYNTHESIS; 140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 090222 - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989); 550700 - Microbiology

Citation Formats

Monot, F, Martin, J R, Petitdemange, H, and Gay, R. Acetone and butanol production by Clostridium acetobutylicum in a synthetic medium. United States: N. p., 1982. Web.
Monot, F, Martin, J R, Petitdemange, H, & Gay, R. Acetone and butanol production by Clostridium acetobutylicum in a synthetic medium. United States.
Monot, F, Martin, J R, Petitdemange, H, and Gay, R. 1982. "Acetone and butanol production by Clostridium acetobutylicum in a synthetic medium". United States.
@article{osti_5792720,
title = {Acetone and butanol production by Clostridium acetobutylicum in a synthetic medium},
author = {Monot, F and Martin, J R and Petitdemange, H and Gay, R},
abstractNote = {The effect of the component concentrations of a synthetic medium on acetone and butanol fermentation by Clostridium acetobutylicum ATCC 824 was investigated. Cell growth was dependent on the presence of Mg, Fe, and K in the medium. Mg and Mn had deleterious effects when in excess. Ammonium acetate in excess caused acid fermentation. The metabolism was composed of two phases: an acid phase and a solvent one. Low concentrations of glucose allowed the first phase only. The theoretical ratio of the conversion of glucose to solvents, which was 28 to 33%, was obtained with the following medium: MgSO/sub 4/, 50 to 200 mg/liter; MnSO/sub 4/, 0 to 20 mg/liter; KCl, 0.015 to 8 g/liter (an equivalent concentration of K+ was supplied in the form of KH/sub 2/PO/sub 4/ and K/sub 2/HPO/sub 4/); FeSO/sub 4/, 1 to 50 mg/liter; ammonium acetate, 1.1 to 2.2 g/liter; para-aminobenzoic acid, 1 mg/liter; biotin, 0.01 mg/liter; glucose, 20 to 60 g/liter. (Refs. 24).},
doi = {},
url = {https://www.osti.gov/biblio/5792720}, journal = {Appl. Environ. Microbiol.; (United States)},
number = ,
volume = 44:6,
place = {United States},
year = {1982},
month = {12}
}