Rapid and efficient capillary column supercritical fluid chromatography with mass spectrometric detection

Journal Article · Thu Nov 01 00:00:00 EST 1984 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00277a048· OSTI ID:5786983

Smith, R D; Kalinoski, H T; Udseth, H R; Wright, B W

Capillary column supercritical fluid chromatography (SFC) using small diameter columns (approx. 50 ..mu..m i.d.) has been demonstrated to provide efficient high-resolution separations. Microparticle packed column SFC has previously been demonstrated to allow very rapid moderate resolution separations, but the large pressure drop required by this approach does not allow effective pressure programming for fast separations to be obtained with capillary column SFC using high-pressure programming rates of up to 50 bar/min and provide advantages compared to packed column methods when the components to be separated span a wide molecular weight range. This approach complements the high-resolution capability of capillary SFC obtained using longer columns and provides increased sensitivity and narrow, nearly constant peak widths ( less than or equal to 6 s), which are ideally compatible with mass spectrometry (MS). Detection limits were found to be approx. 0.1 pg for a series of biphenyl compounds using single ion monitoring. The application of capillary SFC/MS for both rapid and high-resolution separations is demonstrated for standard mixtures and a complex coal tar extract.

• Research Organization: Pacific Northwest Lab., Richland, WA
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 5786983
• Journal Information: Anal. Chem.; (United States), Vol. 56:13
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
01 COAL, LIGNITE, AND PEAT
BIPHENYL
MASS SPECTROSCOPY
SUPERCRITICAL FLUID CHROMATOGRAPHY
COAL TAR OILS
QUANTITATIVE CHEMICAL ANALYSIS
CAPILLARIES
EXPERIMENTAL DATA
PRESSURE DEPENDENCE
TRACE AMOUNTS
AROMATICS
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DATA
HYDROCARBONS
INFORMATION
NUMERICAL DATA
OILS
ORGANIC COMPOUNDS
ORGANS
OTHER ORGANIC COMPOUNDS
SEPARATION PROCESSES
SPECTROSCOPY
400105* - Separation Procedures
400104 - Spectral Procedures- (-1987)
010600 - Coal
Lignite
& Peat- Properties & Composition