Immunocytochemical localization of estrogen receptors in the normal male and female canine urinary tract and prostate
We have used the monoclonal estrogen receptor (ER) antibody H222Sp gamma to localize ER by immunocytochemistry in frozen sections of the normal canine urinary tract of both sexes and of the normal prostate of the male. Striking regional heterogeneity of ER location was observed. In the urinary tract, specific ER staining was confined to nuclei of the transitional epithelium (mucosa) and subjacent stroma (submucosa) of the prostatic urethra in the male dog and of the proximal urethra in the female dog. In both sexes there was a gradient of ER staining intensity along these urethral segments. In the male, ER staining intensity was highest in the region of the verumontanum. The pattern and intensity of staining were similar in the male prostatic urethra and female proximal urethra, indicating a similar concentration of ER in these tissues, which have the same embryological origin. No specific staining was found in the kidney, ureter, bladder, or distal urethra of either sex. In the normal prostate, specific immunocytochemical ER staining was confined to nuclei of the prostatic stroma and prostatic ductal epithelium. Specific staining intensity appeared to be higher in the periurethral region of the prostate than in the periphery. No specific staining was found in the acinar epithelium of the prostate. Based on overall staining intensity there appeared to be a higher concentration of ER in the urethra than in the prostate. Scatchard analysis of (/sup 3/H)estradiol binding confirmed a similar ER content in the urethra of male and female dogs and a higher ER content in the prostatic urethra than in the prostate itself (P less than 0.001).
- Research Organization:
- Johns Hopkins Univ. School of Medicine, Baltimore, MD
- OSTI ID:
- 5786500
- Journal Information:
- Endocrinology; (United States), Vol. 121:5
- Country of Publication:
- United States
- Language:
- English
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ESTROGENS
RECEPTORS
BIOLOGICAL LOCALIZATION
AUTORADIOGRAPHY
BLADDER
CELL NUCLEI
CYTOLOGICAL TECHNIQUES
DOGS
EPITHELIUM
ESTRADIOL
MONOCLONAL ANTIBODIES
PROSTATE
STAINS
TRITIUM COMPOUNDS
URINARY TRACT
ANIMAL TISSUES
ANIMALS
ANTIBODIES
BODY
CELL CONSTITUENTS
ESTRANES
GLANDS
HORMONES
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
MALE GENITALS
MAMMALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
STEROID HORMONES
STEROIDS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques