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Title: D-(/sup 3/H)aspartate retrograde labelling of callosal and association neurons of somatosensory areas I and II of cats

Abstract

Experiments were carried out on cats to ascertain whether corticocortical neurons of somatosensory areas I (SI) and II (SII) could be labelled by retrograde axonal transport of D-(/sup 3/H)aspartate (D-(/sup 3/H)Asp). This tritiated enantiomer of the amino acid aspartate is (1) taken up selectively by axon terminals of neurons releasing aspartate and/or glutamate as excitatory neurotransmitter, (2) retrogradely transported and accumulated in perikarya, (3) not metabolized, and (4) visualized by autoradiography. A solution of D-(/sup 3/H)Asp was injected in eight cats in the trunk and forelimb zones of SI (two cats) or in the forelimb zone of SII (six cats). In order to compare the labelling patterns obtained with D-(/sup 3/H)Asp with those resulting after injection of a nonselective neuronal tracer, horseradish peroxidase (HRP) was delivered mixed with the radioactive tracer in seven of the eight cats. Furthermore, six additional animals received HRP injections in SI (three cats; trunk and forelimb zones) or SII (three cats; forelimb zone). D-(/sup 3/H)Asp retrograde labelling of perikarya was absent from the ipsilateral thalamus of all cats injected with the radioactive tracer but a dense terminal plexus of anterogradely labelled corticothalamic fibers from SI and SII was observed, overlapping the distribution area of thalamocorticalmore » neurons retrogradely labelled with HRP from the same areas. D-(/sup 3/H)Asp-labelled neurones were present in ipsilateral SII (SII-SI association neurones) in cats injected in SI. In these animals a bundle of radioactive fibres was observed in the rostral portion of the corpus callosum entering the contralateral hemisphere. There, neurones retrogradely labelled with silver grains were present in SI (SI-SI callosal neurons).« less

Authors:
; ; ;
Publication Date:
Research Org.:
Univ. of Ancona, Italy
OSTI Identifier:
5784324
Resource Type:
Journal Article
Journal Name:
J. Comp. Neurol.; (United States)
Additional Journal Information:
Journal Volume: 263:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ASPARTIC ACID; MEMBRANE TRANSPORT; NERVE CELLS; LABELLING; AUTORADIOGRAPHY; CATS; NEUROREGULATORS; PEROXIDASES; THALAMUS; TRITIUM COMPOUNDS; AMINO ACIDS; ANIMAL CELLS; ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENTS; BODY; BRAIN; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM; DRUGS; ENZYMES; LABELLED COMPOUNDS; MAMMALS; NERVOUS SYSTEM; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; OXIDOREDUCTASES; SOMATIC CELLS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Barbaresi, P, Fabri, M, Conti, F, and Manzoni, T. D-(/sup 3/H)aspartate retrograde labelling of callosal and association neurons of somatosensory areas I and II of cats. United States: N. p., 1987. Web. doi:10.1002/cne.902630202.
Barbaresi, P, Fabri, M, Conti, F, & Manzoni, T. D-(/sup 3/H)aspartate retrograde labelling of callosal and association neurons of somatosensory areas I and II of cats. United States. https://doi.org/10.1002/cne.902630202
Barbaresi, P, Fabri, M, Conti, F, and Manzoni, T. Tue . "D-(/sup 3/H)aspartate retrograde labelling of callosal and association neurons of somatosensory areas I and II of cats". United States. https://doi.org/10.1002/cne.902630202.
@article{osti_5784324,
title = {D-(/sup 3/H)aspartate retrograde labelling of callosal and association neurons of somatosensory areas I and II of cats},
author = {Barbaresi, P and Fabri, M and Conti, F and Manzoni, T},
abstractNote = {Experiments were carried out on cats to ascertain whether corticocortical neurons of somatosensory areas I (SI) and II (SII) could be labelled by retrograde axonal transport of D-(/sup 3/H)aspartate (D-(/sup 3/H)Asp). This tritiated enantiomer of the amino acid aspartate is (1) taken up selectively by axon terminals of neurons releasing aspartate and/or glutamate as excitatory neurotransmitter, (2) retrogradely transported and accumulated in perikarya, (3) not metabolized, and (4) visualized by autoradiography. A solution of D-(/sup 3/H)Asp was injected in eight cats in the trunk and forelimb zones of SI (two cats) or in the forelimb zone of SII (six cats). In order to compare the labelling patterns obtained with D-(/sup 3/H)Asp with those resulting after injection of a nonselective neuronal tracer, horseradish peroxidase (HRP) was delivered mixed with the radioactive tracer in seven of the eight cats. Furthermore, six additional animals received HRP injections in SI (three cats; trunk and forelimb zones) or SII (three cats; forelimb zone). D-(/sup 3/H)Asp retrograde labelling of perikarya was absent from the ipsilateral thalamus of all cats injected with the radioactive tracer but a dense terminal plexus of anterogradely labelled corticothalamic fibers from SI and SII was observed, overlapping the distribution area of thalamocortical neurons retrogradely labelled with HRP from the same areas. D-(/sup 3/H)Asp-labelled neurones were present in ipsilateral SII (SII-SI association neurones) in cats injected in SI. In these animals a bundle of radioactive fibres was observed in the rostral portion of the corpus callosum entering the contralateral hemisphere. There, neurones retrogradely labelled with silver grains were present in SI (SI-SI callosal neurons).},
doi = {10.1002/cne.902630202},
url = {https://www.osti.gov/biblio/5784324}, journal = {J. Comp. Neurol.; (United States)},
number = ,
volume = 263:2,
place = {United States},
year = {1987},
month = {9}
}