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Title: DNA repair in xeroderma pigmentosum cells treated with combinations of ultraviolet radiation and N-acetoxy-2-acetylaminofluorene

Abstract

Three techniques were used to examine excision repair in human cells treated with ultraviolet radiation, N-acetoxy-2-acetylaminofluorene, and a combination of the two. The three techniques gave similar results. Two types of human cells were used: (a) excision repair proficient (normal human fibroblasts and xeroderma pigmentosum variants); and (b) excision repair deficient (xeroderma pigmentosum, C, D, and E). Saturation doses were determined and used for combined treatments with both agents. We observed two patterns of repair: (a) in repair-proficient cells total repair was additive; and (b) in repair-deficient cells total repair was much less than additive (usually less than that observed for separate treatments) and N-acetocy-2-acetyl-aminofluorene inhibited excision of pyrimidine dimers. We conclude that, in the first group of cells, pathways for repair of ultraviolet radiation- and N-acetoxy-2-acetylaminofluorene-induced lesions are not identical and, in the second group of cells, there is an inhibitory effect exerted by major or minor products of each agent on the repair enzyme(s) of the other.

Authors:
;
Publication Date:
Research Org.:
Brookhaven National Lab., Upton, NY
OSTI Identifier:
5775415
Resource Type:
Journal Article
Journal Name:
Cancer Res.; (United States)
Additional Journal Information:
Journal Volume: 39
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA; BIOLOGICAL REPAIR; XERODERMA PIGMENTOSUM; ACETYL RADICALS; ANIMAL CELLS; ENZYME INHIBITORS; FIBROBLASTS; FLUORENE; MAN; PYRIMIDINES; SYNERGISM; ULTRAVIOLET RADIATION; ACYL RADICALS; ANIMALS; AROMATICS; AZINES; BIOLOGICAL RECOVERY; CONDENSED AROMATICS; CONNECTIVE TISSUE CELLS; DISEASES; ELECTROMAGNETIC RADIATION; HETEROCYCLIC COMPOUNDS; HYDROCARBONS; MAMMALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PRIMATES; RADIATIONS; RADICALS; RECOVERY; REPAIR; SKIN DISEASES; SOMATIC CELLS; VERTEBRATES; 550200* - Biochemistry; 550300 - Cytology; 560400 - Other Environmental Pollutant Effects; 560301 - Chemicals Metabolism & Toxicology- Cells- (-1987)

Citation Formats

Ahmed, F.E., and Setlow, R.B. DNA repair in xeroderma pigmentosum cells treated with combinations of ultraviolet radiation and N-acetoxy-2-acetylaminofluorene. United States: N. p., 1979. Web.
Ahmed, F.E., & Setlow, R.B. DNA repair in xeroderma pigmentosum cells treated with combinations of ultraviolet radiation and N-acetoxy-2-acetylaminofluorene. United States.
Ahmed, F.E., and Setlow, R.B. Thu . "DNA repair in xeroderma pigmentosum cells treated with combinations of ultraviolet radiation and N-acetoxy-2-acetylaminofluorene". United States.
@article{osti_5775415,
title = {DNA repair in xeroderma pigmentosum cells treated with combinations of ultraviolet radiation and N-acetoxy-2-acetylaminofluorene},
author = {Ahmed, F.E. and Setlow, R.B.},
abstractNote = {Three techniques were used to examine excision repair in human cells treated with ultraviolet radiation, N-acetoxy-2-acetylaminofluorene, and a combination of the two. The three techniques gave similar results. Two types of human cells were used: (a) excision repair proficient (normal human fibroblasts and xeroderma pigmentosum variants); and (b) excision repair deficient (xeroderma pigmentosum, C, D, and E). Saturation doses were determined and used for combined treatments with both agents. We observed two patterns of repair: (a) in repair-proficient cells total repair was additive; and (b) in repair-deficient cells total repair was much less than additive (usually less than that observed for separate treatments) and N-acetocy-2-acetyl-aminofluorene inhibited excision of pyrimidine dimers. We conclude that, in the first group of cells, pathways for repair of ultraviolet radiation- and N-acetoxy-2-acetylaminofluorene-induced lesions are not identical and, in the second group of cells, there is an inhibitory effect exerted by major or minor products of each agent on the repair enzyme(s) of the other.},
doi = {},
journal = {Cancer Res.; (United States)},
number = ,
volume = 39,
place = {United States},
year = {1979},
month = {2}
}