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Title: Diminished respirative growth and enhanced assimilative sugar uptake result in higher specific fermentation rates by the mutant Pichia stipitis FPL-061

Abstract

A mutant strain of Pichia stipitis, FPL-061, was obtained by selecting for growth on L-xylose in the presence of respiratory inhibitors. The specific fermentation rate of FPL-061, was higher than that of the parent, Pichia stipitis CBS 6054, because of its lower cell yield and growth rate and higher specific substrate uptake rate. With a mixture of glucose and xylose, the mutant strain FPL-061 produced 29.4 g ethanol/L with a yield of 0.42 g ethanol/g sugar consumed. By comparison, CBS 6054 produced 25.7 g ethanol/L with a yield of 0.35 g/g. The fermentation was most efficient at an aeration rate of 9.2 mmoles O{sub 2} L{sup -1} h{sup -1}. At high aeration rates (22 mmoles O{sub 2} L{sup -1} h{sup -1}), the mutant cell yield was less than that of the parent. At low aeration rates, (1.1 to 2.5 O{sub 2} L{sup -1} h{sup -1}), cell yields were similar, the ethanol formation rates were low, and xylitol accumulation was observed in both the strains. Both strains respired the ethanol once sugar was exhausted. We infer from the results that the mutant, P. stipitis FPL-061, diverts a larger fraction of its metabolic energy from cell growth into ethanol production. 21 refs.,more » 5 figs.« less

Authors:
 [1];  [1]
  1. Forest Products Lab., Madison, WI (United States)
Publication Date:
OSTI Identifier:
576215
Report Number(s):
CONF-960539-
Journal ID: ABIBDL; ISSN 0273-2289; TRN: 98:000980-0007
Resource Type:
Journal Article
Journal Name:
Applied Biochemistry and Biotechnology
Additional Journal Information:
Journal Volume: 63-65; Conference: 18. symposium on biotechnology for fuels and chemicals, Gatlinburg, TN (United States), 5-9 May 1996; Other Information: PBD: Spr 1997
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; AERATION; ETHANOL; FERMENTATION; GLUCOSE; UPTAKE; YIELDS; XYLOSE; MUTANTS; METABOLISM; BIOMASS

Citation Formats

Sreenath, H K, Jeffries, T W, and Univ. of Wisconsin, Madison, WI. Diminished respirative growth and enhanced assimilative sugar uptake result in higher specific fermentation rates by the mutant Pichia stipitis FPL-061. United States: N. p., 1997. Web. doi:10.1007/BF02920417.
Sreenath, H K, Jeffries, T W, & Univ. of Wisconsin, Madison, WI. Diminished respirative growth and enhanced assimilative sugar uptake result in higher specific fermentation rates by the mutant Pichia stipitis FPL-061. United States. doi:10.1007/BF02920417.
Sreenath, H K, Jeffries, T W, and Univ. of Wisconsin, Madison, WI. Wed . "Diminished respirative growth and enhanced assimilative sugar uptake result in higher specific fermentation rates by the mutant Pichia stipitis FPL-061". United States. doi:10.1007/BF02920417.
@article{osti_576215,
title = {Diminished respirative growth and enhanced assimilative sugar uptake result in higher specific fermentation rates by the mutant Pichia stipitis FPL-061},
author = {Sreenath, H K and Jeffries, T W and Univ. of Wisconsin, Madison, WI},
abstractNote = {A mutant strain of Pichia stipitis, FPL-061, was obtained by selecting for growth on L-xylose in the presence of respiratory inhibitors. The specific fermentation rate of FPL-061, was higher than that of the parent, Pichia stipitis CBS 6054, because of its lower cell yield and growth rate and higher specific substrate uptake rate. With a mixture of glucose and xylose, the mutant strain FPL-061 produced 29.4 g ethanol/L with a yield of 0.42 g ethanol/g sugar consumed. By comparison, CBS 6054 produced 25.7 g ethanol/L with a yield of 0.35 g/g. The fermentation was most efficient at an aeration rate of 9.2 mmoles O{sub 2} L{sup -1} h{sup -1}. At high aeration rates (22 mmoles O{sub 2} L{sup -1} h{sup -1}), the mutant cell yield was less than that of the parent. At low aeration rates, (1.1 to 2.5 O{sub 2} L{sup -1} h{sup -1}), cell yields were similar, the ethanol formation rates were low, and xylitol accumulation was observed in both the strains. Both strains respired the ethanol once sugar was exhausted. We infer from the results that the mutant, P. stipitis FPL-061, diverts a larger fraction of its metabolic energy from cell growth into ethanol production. 21 refs., 5 figs.},
doi = {10.1007/BF02920417},
journal = {Applied Biochemistry and Biotechnology},
number = ,
volume = 63-65,
place = {United States},
year = {1997},
month = {12}
}