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Title: Effect of postnatal lead exposure on the development of sympathetic innervation of the heart. [Rats]

Abstract

To determine possible mechanisms for this Pb-induced cardiotoxicity, several neutrochemical parameters indicative of cardiac sympathetic innervation were measured in developing rats. Presynaptic indices of nerve terminal development which were studied included steady-state levels of norepinephrine, neuronal uptake and vesicular storage of /sup 3/H-norepinephrine. Analysis of postsynaptic development was accomplished by quantitating the density of ..beta..-adrenergic receptors and by measuring the activity of adenylate cyclase. Rat pups were exposed to Pb from birth to weaning (21 days) via the milk of dams whose drinking water contained 0.2% Pb acetate. This method and level of Pb treatment had no effect on body or heart weight development, however, it did result in a seven-fold increase in the blood Pb content (70-75 ..mu..g/dl) of the treated pups during the period of exposure. Pb exposure accelerated the development of sympathetic innervation of the heart as detected by significant increases in the vesicular uptake of /sup 3/H-norepinephrine and the steady-state concentration of norepinephrine measured at postnatal day 4. On the other hand, ontogeny of the neutronal uptake of /sup 3/H-norepinephrine in the heart and in the forebrain was not affected by Pb treatment. The apparent premature development of sympathetic innervation induced by Pb treatment was notmore » reflected in significant alterations in either the density or the affinity of ..beta..-adrenergic receptor sites determined by the binding kinetics of /sup 3/H-dihydroalprenolol.« less

Authors:
Publication Date:
OSTI Identifier:
5762133
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D.)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LEAD; BIOLOGICAL EFFECTS; NORADRENALINE; UPTAKE; TRITIUM COMPOUNDS; ACETATES; AUTONOMIC NERVOUS SYSTEM; DRINKING WATER; HEART; MILK; NEONATES; NERVES; RATS; ADRENAL HORMONES; ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENTS; BIOLOGICAL MATERIALS; BODY; BODY FLUIDS; CARBOXYLIC ACID SALTS; CARDIOTONICS; CARDIOVASCULAR AGENTS; CARDIOVASCULAR SYSTEM; DRUGS; ELEMENTS; FOOD; HORMONES; HYDROGEN COMPOUNDS; LABELLED COMPOUNDS; MAMMALS; MATERIALS; METALS; NERVOUS SYSTEM; NEUROREGULATORS; ORGANS; OXYGEN COMPOUNDS; RODENTS; STEROID HORMONES; SYMPATHOMIMETICS; VERTEBRATES; WATER 560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)

Citation Formats

Abreu, M.E.. Effect of postnatal lead exposure on the development of sympathetic innervation of the heart. [Rats]. United States: N. p., 1983. Web.
Abreu, M.E.. Effect of postnatal lead exposure on the development of sympathetic innervation of the heart. [Rats]. United States.
Abreu, M.E.. 1983. "Effect of postnatal lead exposure on the development of sympathetic innervation of the heart. [Rats]". United States. doi:.
@article{osti_5762133,
title = {Effect of postnatal lead exposure on the development of sympathetic innervation of the heart. [Rats]},
author = {Abreu, M.E.},
abstractNote = {To determine possible mechanisms for this Pb-induced cardiotoxicity, several neutrochemical parameters indicative of cardiac sympathetic innervation were measured in developing rats. Presynaptic indices of nerve terminal development which were studied included steady-state levels of norepinephrine, neuronal uptake and vesicular storage of /sup 3/H-norepinephrine. Analysis of postsynaptic development was accomplished by quantitating the density of ..beta..-adrenergic receptors and by measuring the activity of adenylate cyclase. Rat pups were exposed to Pb from birth to weaning (21 days) via the milk of dams whose drinking water contained 0.2% Pb acetate. This method and level of Pb treatment had no effect on body or heart weight development, however, it did result in a seven-fold increase in the blood Pb content (70-75 ..mu..g/dl) of the treated pups during the period of exposure. Pb exposure accelerated the development of sympathetic innervation of the heart as detected by significant increases in the vesicular uptake of /sup 3/H-norepinephrine and the steady-state concentration of norepinephrine measured at postnatal day 4. On the other hand, ontogeny of the neutronal uptake of /sup 3/H-norepinephrine in the heart and in the forebrain was not affected by Pb treatment. The apparent premature development of sympathetic innervation induced by Pb treatment was not reflected in significant alterations in either the density or the affinity of ..beta..-adrenergic receptor sites determined by the binding kinetics of /sup 3/H-dihydroalprenolol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = 1983,
month = 1
}

Thesis/Dissertation:
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  • Pups from female rats exposed to 40-to-80 mg of lead per liter in their drinking water (low-lead group) and 160-to-320 mg of lead per liter water (high-lead group) were examined at 1 to 18 days of age. Maximal electroshock seizure (MES) patterns were determined and, upon recovery, whole blood, plasma, cerebrospinal fluid (CSF) and cerebral cortex samples were collected. Approximately one-half of the pups was used to determine the cerebral cortical extracellular water space (ECS). The other half was used to determine whole blood lead concentrations, plasma electrolytes (Na/sup +/, K/sup +/ and Cl/sup -/), CSF electrolytes, and cerebral corticalmore » lead content, electrolytes, total water spaces, protein content, DNA content, carbonic anhydrase (CA) activity, and sodium- potassium-activated adenosinetriphosphatase (Na/sup +//K/sup +/-ATPase) activity. Neither the low- nor the high-lead groups had significant changes in body weight, body length, hematocrit or cerebral wet weight at any age studied. Whole blood and cerebral cortical lead contents were increased, dose-dependently, at each day of age. Hyperexcitability as measured by MES was observed in lead-exposed pups at 6, 9 and 12 days of age. These observations demonstrate that prenatal and postnatal exposure to lead causes increased susceptibility to MES and alterations in normal developmental patterns of the cerebral cortex. Such alterations appear to result from the greater vulnerability of the glial population to the adverse effects of lead than are neutrons. Thus, effects on the glia can account for the electrolyte imbalances, cellular edema and hyperexcitability resulting from exposure to lead.« less
  • Groups of laboratory rats were subcutaneously inoculated with the infective stage larvae of the rate nematode, Nippostrongylus brasiliensis, irradiated with Co/sup 60/ at dose levels of 10,000, 20,000, 30,000, 40,000, 50,000, 60,000, radiation on larval growth and development, and the degree of immunity produced by rats when inoculated with larvae irradiated at various dose leveis and subsequently challenged with normal larvae. Data obtained from four experiments revealed that: there was a marked decrease in the growth rate of the larvae, egg production of the adult worms, and the worm burden in rats as the graded doses of radiation administered tomore » the larvae were increased from 10,000 to 100,000r, when rats were inoculated with larvae irradiated with 115,000r, the larvae that survived had not developed in size when recovered from rats 9 days later, larvae exposed to radiation doses up to 50,000r showed no apparent alterations of their internal structures upon necropsy 10 days after inoculation, and rats inoculated with larvae irradiated at various dose levels were as effective as normal larvae in stimulating resistance to reinfection. (Dissertation Abstr. 23: May 1963)« less