Recombinant Brucella abortus gene expressing immunogenic protein
This patent describes a synthetic recombinant DNA molecule containing a DNA sequence. It comprises a gene of Brucella abortus encoding an immunogenic protein having a molecular weight of approximately 31,000 daltons as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis under denaturing conditions, the protein having an isoelectric point around 4.9, and containing a twenty-five amino acid sequence from its amino terminal end consisting of Gln-Ala-Pro-Thr-Phe-Phe-Arg-Ile-Gly-Thr-Gly-Gly-Thr-Ala-Gly-Thr-Tyr-Tyr-Pro-Ile-Gly-Gly-Leu-Ile-Ala, wherein Gln, Ala, Pro, Thr, Phe, Arg, Ile, Gly, Tyr, and Leu, respectively, represent glutamine, alanine, proline, threonine, phenylalanine, arginine, isolecuine, glycine, tyrosine, and leucine.
- Assignee:
- Iowa State Univ. Research Foundation, Ames, IA (United States)
- Patent Number(s):
- US 5023174; A
- Application Number:
- PPN: US 7-365226
- OSTI ID:
- 5755016
- Resource Relation:
- Patent File Date: 12 Jun 1989
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BRUCELLA
PHENOTYPE
PROTEIN ENGINEERING
RECOMBINANT DNA
DNA SEQUENCING
BIOSYNTHESIS
ELECTROPHORESIS
GELS
GENE RECOMBINATION
IMMUNOGLOBULINS
IMMUNOLOGY
BACTERIA
COLLOIDS
DISPERSIONS
DNA
GLOBULINS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
550400* - Genetics