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Title: Recombinant Brucella abortus gene expressing immunogenic protein

Abstract

This patent describes a synthetic recombinant DNA molecule containing a DNA sequence. It comprises a gene of Brucella abortus encoding an immunogenic protein having a molecular weight of approximately 31,000 daltons as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis under denaturing conditions, the protein having an isoelectric point around 4.9, and containing a twenty-five amino acid sequence from its amino terminal end consisting of Gln-Ala-Pro-Thr-Phe-Phe-Arg-Ile-Gly-Thr-Gly-Gly-Thr-Ala-Gly-Thr-Tyr-Tyr-Pro-Ile-Gly-Gly-Leu-Ile-Ala, wherein Gln, Ala, Pro, Thr, Phe, Arg, Ile, Gly, Tyr, and Leu, respectively, represent glutamine, alanine, proline, threonine, phenylalanine, arginine, isolecuine, glycine, tyrosine, and leucine.

Inventors:
;
Publication Date:
OSTI Identifier:
5755016
Patent Number(s):
US 5023174; A
Application Number:
PPN: US 7-365226
Assignee:
Iowa State Univ. Research Foundation, Ames, IA (United States) NOV; NOV-92-011031; EDB-92-043539
Resource Type:
Patent
Resource Relation:
Patent File Date: 12 Jun 1989
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BRUCELLA; PHENOTYPE; PROTEIN ENGINEERING; RECOMBINANT DNA; DNA SEQUENCING; BIOSYNTHESIS; ELECTROPHORESIS; GELS; GENE RECOMBINATION; IMMUNOGLOBULINS; IMMUNOLOGY; BACTERIA; COLLOIDS; DISPERSIONS; DNA; GLOBULINS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PROTEINS; STRUCTURAL CHEMICAL ANALYSIS; SYNTHESIS; 550400* - Genetics

Citation Formats

Mayfield, J.E., and Tabatabai, L.B. Recombinant Brucella abortus gene expressing immunogenic protein. United States: N. p., 1991. Web.
Mayfield, J.E., & Tabatabai, L.B. Recombinant Brucella abortus gene expressing immunogenic protein. United States.
Mayfield, J.E., and Tabatabai, L.B. Tue . "Recombinant Brucella abortus gene expressing immunogenic protein". United States.
@article{osti_5755016,
title = {Recombinant Brucella abortus gene expressing immunogenic protein},
author = {Mayfield, J.E. and Tabatabai, L.B.},
abstractNote = {This patent describes a synthetic recombinant DNA molecule containing a DNA sequence. It comprises a gene of Brucella abortus encoding an immunogenic protein having a molecular weight of approximately 31,000 daltons as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis under denaturing conditions, the protein having an isoelectric point around 4.9, and containing a twenty-five amino acid sequence from its amino terminal end consisting of Gln-Ala-Pro-Thr-Phe-Phe-Arg-Ile-Gly-Thr-Gly-Gly-Thr-Ala-Gly-Thr-Tyr-Tyr-Pro-Ile-Gly-Gly-Leu-Ile-Ala, wherein Gln, Ala, Pro, Thr, Phe, Arg, Ile, Gly, Tyr, and Leu, respectively, represent glutamine, alanine, proline, threonine, phenylalanine, arginine, isolecuine, glycine, tyrosine, and leucine.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jun 11 00:00:00 EDT 1991},
month = {Tue Jun 11 00:00:00 EDT 1991}
}