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Title: Energetics of end product excretion in anaerobic bacteria and the metabolism of fatty acids by Syntrophomonas wolfei: Progress report, November 16, 1986-November 15, 1987

Abstract

We have studied the growth and metabolism of Syntrophomonas wolfei in pure culture with crotonate as the energy source. S. wolfei grows in crotonate mineral salts medium without rumen fluid with cobalamin, thymine, lipoic acid and biotin added. However, after four to six transfers in this medium, growth ceases, indicating that another vitamin is required. The chemically defined medium allows large batches of S. wolfei to be grown for enzyme purification. All the enzymes involved in the oxidation of crotonyl-CoA to acetate have been detected. The pure culture of S. wolfei or coculture of S. wolfei grown with crotonate contain high activities of a crotonate: acetyl-CoA CoA-transferase activity. This activity is not detected in cocultures grown with butyrate. Thus, we believe that the reason why S. wolfei can now grow with crotonate is that an alteration or mutation occurred which allows the organism to activate this crotonate. S. wolfei also makes small amounts of H/sub 2/ when grown in pure culture with crotonate. A methyl viologen-dependent hydrogenase activity was found. We have also demonstrated the production of H/sub 2/ from 3-hydroxybutyryl-CoA in cell-free extracts of S. wolfei by coupling H/sub 2/ production to CH/sub 4/ production with the addition ofmore » Methanobacterium bryantii and directly using a hydrogen electrode. These results clearly show that S. wolfei makes H/sub 2/. S. wolfei does not contain formate dehydrogenase or CO dehydrogenase activities.« less

Authors:
Publication Date:
Research Org.:
Oklahoma Univ., Norman (USA)
OSTI Identifier:
5748513
Report Number(s):
DOE/ER/13053-6
ON: DE88002790
DOE Contract Number:  
AS05-83ER13053
Resource Type:
Technical Report
Resource Relation:
Other Information: Portions of this document are illegible in microfiche products
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; CROTONIC ACID; BIOSYNTHESIS; CATABOLISM; LABELLING; OXIDOREDUCTASES; FRACTIONATION; ALGAE; ANAEROBIC DIGESTION; CARBON 14 COMPOUNDS; HYDROGENASES; PROGRESS REPORT; TRACER TECHNIQUES; BIOCONVERSION; CARBOXYLIC ACIDS; DIGESTION; DOCUMENT TYPES; ENZYMES; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MANAGEMENT; METABOLISM; MONOCARBOXYLIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; PLANTS; PROCESSING; SEPARATION PROCESSES; SYNTHESIS; WASTE MANAGEMENT; WASTE PROCESSING; 140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 550500 - Metabolism

Citation Formats

McInerney, M J. Energetics of end product excretion in anaerobic bacteria and the metabolism of fatty acids by Syntrophomonas wolfei: Progress report, November 16, 1986-November 15, 1987. United States: N. p., 1987. Web.
McInerney, M J. Energetics of end product excretion in anaerobic bacteria and the metabolism of fatty acids by Syntrophomonas wolfei: Progress report, November 16, 1986-November 15, 1987. United States.
McInerney, M J. Thu . "Energetics of end product excretion in anaerobic bacteria and the metabolism of fatty acids by Syntrophomonas wolfei: Progress report, November 16, 1986-November 15, 1987". United States.
@article{osti_5748513,
title = {Energetics of end product excretion in anaerobic bacteria and the metabolism of fatty acids by Syntrophomonas wolfei: Progress report, November 16, 1986-November 15, 1987},
author = {McInerney, M J},
abstractNote = {We have studied the growth and metabolism of Syntrophomonas wolfei in pure culture with crotonate as the energy source. S. wolfei grows in crotonate mineral salts medium without rumen fluid with cobalamin, thymine, lipoic acid and biotin added. However, after four to six transfers in this medium, growth ceases, indicating that another vitamin is required. The chemically defined medium allows large batches of S. wolfei to be grown for enzyme purification. All the enzymes involved in the oxidation of crotonyl-CoA to acetate have been detected. The pure culture of S. wolfei or coculture of S. wolfei grown with crotonate contain high activities of a crotonate: acetyl-CoA CoA-transferase activity. This activity is not detected in cocultures grown with butyrate. Thus, we believe that the reason why S. wolfei can now grow with crotonate is that an alteration or mutation occurred which allows the organism to activate this crotonate. S. wolfei also makes small amounts of H/sub 2/ when grown in pure culture with crotonate. A methyl viologen-dependent hydrogenase activity was found. We have also demonstrated the production of H/sub 2/ from 3-hydroxybutyryl-CoA in cell-free extracts of S. wolfei by coupling H/sub 2/ production to CH/sub 4/ production with the addition of Methanobacterium bryantii and directly using a hydrogen electrode. These results clearly show that S. wolfei makes H/sub 2/. S. wolfei does not contain formate dehydrogenase or CO dehydrogenase activities.},
doi = {},
url = {https://www.osti.gov/biblio/5748513}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1987},
month = {1}
}

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