Growth of Acinetobacter sp. strain HO1-N on n-hexadecanol: physiological and ultrastructural characteristics

Journal Article · Mon Apr 01 00:00:00 EST 1985 · J. Bacteriol.; (United States)

OSTI ID:5748389

Singer, M E; Tyler, S M; Finnerty, W R

The growth of Acinetobacter sp. strain HO1-N on hexadecanol results in the formation of intracytoplasmic membranes and intracellular rectangular inclusions containing one of the end products of hexadecanol metabolism, hexadecyl palmitate. The intracellular inclusions were purified and characterized as wax ester inclusions consisting of 85.6% hexadecyl palmitate, 4.8% hexadecanol, and 9.6% phospholipid, with a phospholipid-to-protein ratio of 0.42 ..mu..mol of lipid phosphate per mg of inclusion protein. The cellular lipids consisted of 69.8% hexadecyl palmitate, 22.8% phospholipid, 1.9% triglyceride, 4.7% mono- and diglyceride, 0.1% free fatty acid, and 0.8% hexadecanol, as compared with 98% hexadecyl palmitate and 1.9% triglyceride, which comprised the extracellular lipids. Cell-associated hexadecanol represented 0.05% of the exogenously supplied hexadecanol, with hexadecyl palmitate accounting for 14.7% of the total cellular dry weight. Acinetobacter sp. strain HO1-N possesses a mechanism for the intracellular packaging of hexadecyl palmitate in wax ester inclusions, which differ in structure and chemical composition from hydrocarbon inclusions isolated from hexadecane-grown cells.

• Research Organization: Univ. of Georgia, Athens
• OSTI ID: 5748389
• Journal Information: J. Bacteriol.; (United States), Vol. 162:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKANES
CHEMICAL COMPOSITION
METABOLISM
BACTERIA
GROWTH
ULTRASTRUCTURAL CHANGES
BIOLOGICAL EFFECTS
LIPIDS
PURIFICATION
HYDROCARBONS
MICROORGANISMS
MORPHOLOGICAL CHANGES
ORGANIC COMPOUNDS
560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)