Determination of hydrogen peroxide concentrations by flow injection analysis based on the enhanced chemiluminescent reaction using peroxidase

Journal Article · Sun Jan 01 00:00:00 EST 1989 · Analytical Letters; (United States)

DOI:https://doi.org/10.1080/00032718908051233· OSTI ID:5731804

Eremin, S A; Vlasenko, S B; Osipov, A P; Eremina, I D; Egerov, A M ^[1]

---

1. M.V. Lomonosov State Univ., Moscow (USSR)

The technique of flow injection analysis was employed in the determination of hydrogen peroxide. The method was based on the chemiluminescence reaction of luminol with H{sub 2}O{sub 2} which is catalyzed by horseradish peroxidase and enhanced by p-iodophenol. Hydrogen peroxide was linearly detected in the range 10{sup {minus}6}M-10{sup {minus}4}M by measuring the maximum intensity of light emitted. The detection limit is about 1 10{sup {minus}6}M hydrogen peroxide. Transition metal cations at millimolar concentrations do not have any interference on the determination of hydrogen peroxide by FIA based on the enhanced chemiluminescent reaction. This technique is relatively rapid and simple, and permits measurement of up to 80 samples/hr using generally available equipment.

• OSTI ID: 5731804
• Journal Information: Analytical Letters; (United States), Vol. 22:9; ISSN 0003-2719
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
HYDROGEN PEROXIDE
CHEMILUMINESCENCE
REDUCTION
CATALYSIS
CHEMICAL REACTIONS
EXPERIMENTAL DATA
LUMINOL
ORGANIC IODINE COMPOUNDS
OXIDATION
PEROXIDASES
PHENOLS
QUANTITATIVE CHEMICAL ANALYSIS
QUANTITY RATIO
SAMPLE PREPARATION
AMINES
AROMATICS
AZINES
CHEMICAL ANALYSIS
DATA
ENZYMES
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INFORMATION
LUMINESCENCE
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PEROXIDES
PHTHALAZINES
PROTEINS
PYRIDAZINES
400102* - Chemical & Spectral Procedures