Antagonistic action of a tumor promoter and a poly(adenosine diphosphoribose) synthesis inhibitor in radiation-induced transformation in vitro
Transformation of mouse C3H 10T1/2 cells by X-irradiation in vitro was blocked by the addition of 1 mM 3-aminobenzamide, an inhibitor of polyadenosine diphosphoribose (poly(ADP-ribose)) synthesis immediately after irradiation. 3-Aminobenzamide also inhibited an increase in the frequency of transformants caused by the addition of the tumor promoter, 12-O-tetradecanoylphorbol-13-acetate, 7 days after irradiation. These results demonstrate a role for poly(ADP-ribose) synthesis during the initiation and promotion stages of transformation. From previous studies it is known that poly(ADP-ribose) synthesis is stimulated by the DNA damage caused by X rays during initiation. During promotion, however, 12-O-tetradecanoylphorbol-13-acetate acted as a mitogen but did not induce detectable DNA damage, and we could detect no stimulation of poly(ADP-ribose) synthetase. The roles of poly(ADP-ribose) during initiation and during promotion must, therefore, be significantly different.
- Research Organization:
- Columbia Univ. College of Physicians and Surgeons, New York, NY
- OSTI ID:
- 5731370
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Vol. 3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMIDES
BIOLOGICAL EFFECTS
ONCOGENIC TRANSFORMATIONS
BIOLOGICAL PATHWAYS
ADP
BIOLOGICAL RADIATION EFFECTS
CARCINOGENS
CLONE CELLS
FIBROBLASTS
INHIBITION
MICE
TUMOR PROMOTERS
X RADIATION
ANIMAL CELLS
ANIMALS
CELL CULTURES
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
MAMMALS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROMOTERS
RADIATION EFFECTS
RADIATIONS
RODENTS
SOMATIC CELLS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)
560301 - Chemicals Metabolism & Toxicology- Cells- (-1987)