Separation of individual sulfated bile acid conjugates as calcium complexes using reversed-phase partition thin-layer chromatography

Journal Article · Mon Jan 01 00:00:00 EST 1979 · J. Lipid Res.; (United States)

OSTI ID:5729781

Raedsch, R; Hofmann, A F; Tserng, K Y

A method for separating individual monosulfated primary bile acid conjugates by reversed-phase partition thin-layer chromatography on octadecyl-bonded silica gel is described. The solvent system is acetonitrile containing calcium, probably as calcium carbamate. Excellent resolution of the 3- and 7-monosulfated glycine conjugates, as well as 3- and 7-monosulfated taurine conjugates of cholic and chenodeoxycholic acids is reported. A convenient class separation of sulfated from nonsulfated primary bile acid conjugates by adsorption thin-layer chromatography on low-polarity silica gel is also described.

• Research Organization: Univ. of California, San Diego
• DOE Contract Number: W-31-109-ENG-38
• OSTI ID: 5729781
• Journal Information: J. Lipid Res.; (United States), Vol. 20
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BILE ACIDS
QUALITATIVE CHEMICAL ANALYSIS
BIOCHEMISTRY
SILICA GEL
SOLVENT PROPERTIES
STRUCTURAL CHEMICAL ANALYSIS
SULFIDATION
THIN-LAYER CHROMATOGRAPHY
ADSORBENTS
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHEMISTRY
CHROMATOGRAPHY
HYDROXY COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
SEPARATION PROCESSES
STEROIDS
STEROLS
550200* - Biochemistry