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Title: Alterations in benzo(A)pyrene metabolism and in vivo binding to hepatic DNA in rats red diets containing menhaden oil

Abstract

Polyunsaturated fatty acids of the omega-6 type have been shown to support the mixed function oxidases (MFO) responsible for carcinogen activation and to promote tumorigenesis in laboratory animals. The omega-3 fatty acids contained in menhaden oil (MO) have been shown to enhance MFO activity and increase the binding of Benzo(a)pyrene (B(a)P) metabolites to calf thymus DNA in an in vitro microsomal system. Rats fed two levels of MO (0.5% and 20%) for 11 days received a single i.p. dose of (/sup 3/H)B(a)P (5 m Ci/kg) dissolved in DMSO. At selected time intervals thereafter rats were killed, blood withdrawn, livers removed and DNA extracted. Hepatic microsomes were recovered from control rats on each diet at the time of B(a)P administration to assess MFO activities. Binding of B(a)P to DNA was higher in rats fed the 20% MO diet suggesting an increased rate of B(a)P activation. Blood levels of B(a)P were elevated at 16 and 24 hours post B(a)P, however no differences in urine concentrations were observed. Elevations in concentration of cytochrome P-450, ethoxycoumarin dealkylase, and glutathione S-transferase suggest that omega-3 fatty acids of menhaden fish oil support MFO related reactions not unlike the omega-6 fatty acids.

Authors:
;
Publication Date:
Research Org.:
Univ. of Georgia, Athens
OSTI Identifier:
5718696
Report Number(s):
CONF-8611204-
Journal ID: CODEN: PSEBA; TRN: 88-006396
Resource Type:
Conference
Resource Relation:
Journal Name: Proc. Soc. Exp. Biol. Med.; (United States); Journal Volume: 184:4; Conference: Annual meeting of the Southeast Section of the Society for Experimental Biology and Medicine, Chapel Hill, NC, USA, 13 Nov 1986
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; BENZOPYRENE; BIOCHEMICAL REACTION KINETICS; METABOLISM; MIXED-FUNCTION OXIDASES; ENZYME ACTIVITY; CARBOXYLIC ACIDS; CYTOCHROMES; DNA; LIVER; METABOLITES; MICROSOMES; RATS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANIMALS; AROMATICS; BODY; CELL CONSTITUENTS; CONDENSED AROMATICS; DIGESTIVE SYSTEM; ENZYMES; GLANDS; HYDROCARBONS; ISOTOPE APPLICATIONS; KINETICS; LABELLED COMPOUNDS; MAMMALS; NUCLEIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANOIDS; ORGANS; OXIDOREDUCTASES; OXYGENASES; PIGMENTS; PROTEINS; REACTION KINETICS; RODENTS; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology; 550501 - Metabolism- Tracer Techniques

Citation Formats

Wade, A.E., and Dharwadkar, S. Alterations in benzo(A)pyrene metabolism and in vivo binding to hepatic DNA in rats red diets containing menhaden oil. United States: N. p., 1987. Web.
Wade, A.E., & Dharwadkar, S. Alterations in benzo(A)pyrene metabolism and in vivo binding to hepatic DNA in rats red diets containing menhaden oil. United States.
Wade, A.E., and Dharwadkar, S. Thu . "Alterations in benzo(A)pyrene metabolism and in vivo binding to hepatic DNA in rats red diets containing menhaden oil". United States.
@article{osti_5718696,
title = {Alterations in benzo(A)pyrene metabolism and in vivo binding to hepatic DNA in rats red diets containing menhaden oil},
author = {Wade, A.E. and Dharwadkar, S.},
abstractNote = {Polyunsaturated fatty acids of the omega-6 type have been shown to support the mixed function oxidases (MFO) responsible for carcinogen activation and to promote tumorigenesis in laboratory animals. The omega-3 fatty acids contained in menhaden oil (MO) have been shown to enhance MFO activity and increase the binding of Benzo(a)pyrene (B(a)P) metabolites to calf thymus DNA in an in vitro microsomal system. Rats fed two levels of MO (0.5% and 20%) for 11 days received a single i.p. dose of (/sup 3/H)B(a)P (5 m Ci/kg) dissolved in DMSO. At selected time intervals thereafter rats were killed, blood withdrawn, livers removed and DNA extracted. Hepatic microsomes were recovered from control rats on each diet at the time of B(a)P administration to assess MFO activities. Binding of B(a)P to DNA was higher in rats fed the 20% MO diet suggesting an increased rate of B(a)P activation. Blood levels of B(a)P were elevated at 16 and 24 hours post B(a)P, however no differences in urine concentrations were observed. Elevations in concentration of cytochrome P-450, ethoxycoumarin dealkylase, and glutathione S-transferase suggest that omega-3 fatty acids of menhaden fish oil support MFO related reactions not unlike the omega-6 fatty acids.},
doi = {},
journal = {Proc. Soc. Exp. Biol. Med.; (United States)},
number = ,
volume = 184:4,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1987},
month = {Thu Jan 01 00:00:00 EST 1987}
}

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