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Title: Microbiology and physiology of anaerobic fermentations of cellulose

Abstract

The biochemistry and physiology of four major groups of anaerobic bacteria involved in the conversion of cellulose to methane or chemical feedstocks are examined. Aspects of metabolism which are relevant to the interactions and bioenergetics of consortia are being studied. Properties of the cellulolytic enzyme cluster of Clostridium thermocellum are investigated. Five different hydrogenases have been characterized in detail from anaerobic bacteria. Genes for different hydrogenases are being cloned and sequenced to determine their structural relationships. The role of metal clusters in activation of H{sub 2} is being investigated, as is the structure and role of metal clusters in formate metabolism. The function of formate in the total synthesis of acetate from CO{sub 2} and the role of this primary in anaerobes will be examined as well. Finally, these enzyme studies will be performed on thermophilic bacteria and new, pertinent species will be isolated. 50 refs., 3 figs., 1 tab.

Authors:
Publication Date:
Research Org.:
Georgia Univ., Athens, GA (United States). Research Foundation
Sponsoring Org.:
USDOE; USDOE, Washington, DC (United States)
OSTI Identifier:
5709984
Report Number(s):
DOE/ER/13614-4
ON: DE91015239
DOE Contract Number:  
FG09-86ER13614
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; ACETATES; BIOSYNTHESIS; AZOTOBACTER; BIOLOGICAL PATHWAYS; CELLULOSE; ENZYMATIC HYDROLYSIS; CLOSTRIDIUM; DESULFOVIBRIO; HYDROGENASES; MOLECULAR STRUCTURE; METHANE; ANAEROBIC DIGESTION; BIOCHEMISTRY; BIOMASS; BIOTECHNOLOGY; DNA SEQUENCING; DNA-CLONING; FORMATES; MOLECULAR BIOLOGY; PROGRESS REPORT; TEMPERATURE DEPENDENCE; ALKANES; BACTERIA; BIOCONVERSION; CARBOHYDRATES; CARBOXYLIC ACID SALTS; CHEMICAL REACTIONS; CHEMISTRY; CLONING; DECOMPOSITION; DIGESTION; DNA HYBRIDIZATION; DOCUMENT TYPES; ENERGY SOURCES; ENZYMES; HYBRIDIZATION; HYDROCARBONS; HYDROLYSIS; LYSIS; MANAGEMENT; MICROORGANISMS; ORGANIC COMPOUNDS; OXIDOREDUCTASES; POLYSACCHARIDES; PROCESSING; RENEWABLE ENERGY SOURCES; SACCHARIDES; SOLVOLYSIS; STRUCTURAL CHEMICAL ANALYSIS; SULFATE-REDUCING BACTERIA; SYNTHESIS; WASTE MANAGEMENT; WASTE PROCESSING; 090900* - Biomass Fuels- Processing- (1990-); 550700 - Microbiology; 550200 - Biochemistry

Citation Formats

Wiegel, J. Microbiology and physiology of anaerobic fermentations of cellulose. United States: N. p., 1991. Web.
Wiegel, J. Microbiology and physiology of anaerobic fermentations of cellulose. United States.
Wiegel, J. 1991. "Microbiology and physiology of anaerobic fermentations of cellulose". United States.
@article{osti_5709984,
title = {Microbiology and physiology of anaerobic fermentations of cellulose},
author = {Wiegel, J},
abstractNote = {The biochemistry and physiology of four major groups of anaerobic bacteria involved in the conversion of cellulose to methane or chemical feedstocks are examined. Aspects of metabolism which are relevant to the interactions and bioenergetics of consortia are being studied. Properties of the cellulolytic enzyme cluster of Clostridium thermocellum are investigated. Five different hydrogenases have been characterized in detail from anaerobic bacteria. Genes for different hydrogenases are being cloned and sequenced to determine their structural relationships. The role of metal clusters in activation of H{sub 2} is being investigated, as is the structure and role of metal clusters in formate metabolism. The function of formate in the total synthesis of acetate from CO{sub 2} and the role of this primary in anaerobes will be examined as well. Finally, these enzyme studies will be performed on thermophilic bacteria and new, pertinent species will be isolated. 50 refs., 3 figs., 1 tab.},
doi = {},
url = {https://www.osti.gov/biblio/5709984}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed May 01 00:00:00 EDT 1991},
month = {Wed May 01 00:00:00 EDT 1991}
}

Technical Report:
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