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Title: Chlorine-35 nuclear magnetic resonance study of zinc-nucleotide triphosphate complexes

Abstract

The interaction of Zn(II) with nucleotide triphosphates has been studied by /sup 35/Cl NMR and by /sup 1/H NMR. In 0.5 M NaCl solutions, enhanced /sup 35/Cl relaxation is produced by the metal ion in a Zn(II)-nucleotide triphosphate complex, Zn(NTP). In equimolar metal-ligand solutions, the adenosine triphosphate complex, Zn(ATP)/sup 2 -/, produces about twice as much /sup 35/Cl relaxation as do the inosine triphoshate or uridine triphosphate complexes, Zn(ITP)/sup 2 -/ and Zn(UTP)/sup 2 -/. /sup 35/Cl relaxation by the cytidine triphosphate complex, Zn(CTP)/sup 2 -/, is intermediate. In each case, the enhanced /sup 35/Cl relaxation found, relative to the Zn/sup 2 +/(aq) value, is removed by interaction with excess nucleotide. In the presence of excess Zn(II) ions, purine nucleotides form complexes which have high /sup 35/Cl molar relaxivity metal ion environments. These observations are discussed in terms of possible structures which could lead to such relaxation environments.

Authors:
;
Publication Date:
Research Org.:
Univ. of California, Livermore
OSTI Identifier:
5707195
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Journal Article
Journal Name:
J. Phys. Chem.; (United States)
Additional Journal Information:
Journal Volume: 83:26
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; NUCLEOTIDES; CHEMICAL REACTIONS; PHOSPHATES; ZINC COMPLEXES; EXPERIMENTAL DATA; ISOLATED VALUES; NUCLEAR MAGNETIC RESONANCE; COMPLEXES; DATA; DATA FORMS; INFORMATION; MAGNETIC RESONANCE; NUMERICAL DATA; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PHOSPHORUS COMPOUNDS; RESONANCE; 550201* - Biochemistry- Tracer Techniques; 550200 - Biochemistry

Citation Formats

Happe, J A, and Ward, R L. Chlorine-35 nuclear magnetic resonance study of zinc-nucleotide triphosphate complexes. United States: N. p., 1979. Web. doi:10.1021/j100489a029.
Happe, J A, & Ward, R L. Chlorine-35 nuclear magnetic resonance study of zinc-nucleotide triphosphate complexes. United States. https://doi.org/10.1021/j100489a029
Happe, J A, and Ward, R L. 1979. "Chlorine-35 nuclear magnetic resonance study of zinc-nucleotide triphosphate complexes". United States. https://doi.org/10.1021/j100489a029.
@article{osti_5707195,
title = {Chlorine-35 nuclear magnetic resonance study of zinc-nucleotide triphosphate complexes},
author = {Happe, J A and Ward, R L},
abstractNote = {The interaction of Zn(II) with nucleotide triphosphates has been studied by /sup 35/Cl NMR and by /sup 1/H NMR. In 0.5 M NaCl solutions, enhanced /sup 35/Cl relaxation is produced by the metal ion in a Zn(II)-nucleotide triphosphate complex, Zn(NTP). In equimolar metal-ligand solutions, the adenosine triphosphate complex, Zn(ATP)/sup 2 -/, produces about twice as much /sup 35/Cl relaxation as do the inosine triphoshate or uridine triphosphate complexes, Zn(ITP)/sup 2 -/ and Zn(UTP)/sup 2 -/. /sup 35/Cl relaxation by the cytidine triphosphate complex, Zn(CTP)/sup 2 -/, is intermediate. In each case, the enhanced /sup 35/Cl relaxation found, relative to the Zn/sup 2 +/(aq) value, is removed by interaction with excess nucleotide. In the presence of excess Zn(II) ions, purine nucleotides form complexes which have high /sup 35/Cl molar relaxivity metal ion environments. These observations are discussed in terms of possible structures which could lead to such relaxation environments.},
doi = {10.1021/j100489a029},
url = {https://www.osti.gov/biblio/5707195}, journal = {J. Phys. Chem.; (United States)},
number = ,
volume = 83:26,
place = {United States},
year = {Thu Dec 27 00:00:00 EST 1979},
month = {Thu Dec 27 00:00:00 EST 1979}
}