Evaluation of rapid radiometric method for drug susceptibility testing of Mycobacterium tuberculosis

Journal Article · Fri May 01 00:00:00 EDT 1981 · J. Clin. Microbiol.; (United States)

OSTI ID:5705537

Siddiqi, S H; Libonati, J P; Middlebrook, G

A total of 106 isolates of Mycobacterium tuberculosis were tested for drug susceptibility by the conventional 7H11 plate method and by a new rapid radiometric method using special 7H12 liquid medium with /sup 14/C-labeled substrate. Results obtained by the two methods were compared for rapidity, sensitivity, and specificity of the new test method. There was 98% overall agreement between the results obtained by the two methods. Of a total of 424 drug tests, only 8 drug results did not agree, mostly in the case of streptomycin. This new procedure was found to be rapid, with 87% of the tests results reportable within 4 days and 98% reportable within 5 days as compared to the usual 3 weeks required with the conventional indirect susceptibility test method. The results of this preliminary study indicate that the rapid radiometric method seems to have the potential for routine laboratory use and merits further investigations.

• OSTI ID: 5705537
• Journal Information: J. Clin. Microbiol.; (United States), Vol. 13:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
CARBON 14 COMPOUNDS
ISOTOPE APPLICATIONS
DRUGS
BIOLOGICAL EFFECTS
MYCOBACTERIUM TUBERCULOSIS
SENSITIVITY
CULTURE MEDIA
MEASURING METHODS
PHARMACOLOGY
SPECIFICITY
BACTERIA
LABELLED COMPOUNDS
MICROORGANISMS
MYCOBACTERIUM
550701* - Microbiology- Tracer Techniques
550601 - Medicine- Unsealed Radionuclides in Diagnostics
550201 - Biochemistry- Tracer Techniques