A damage-responsive DNA binding protein regulates transcription of the yeast DNA repair gene PHR1
- Univ. of North Carolina, Chapel Hill (United States)
The PHR1 gene of Saccharomyces cerevisiae encodes the DNA repair enzyme photolyase. Transcription of PHR1 increases in response to treatment of cells with 254-nm radiation and chemical agents that damage DNA. The authors here the identification of a damage-responsive DNA binding protein, termed photolyase regulatory protein (PRP), and its cognate binding site, termed the PHR1 transcription after DNA damage. PRP activity, monitored by electrophoretic-mobility-shift assay, was detected in cells during normal growth but disappeared within 30 min after irradiation. Copper-phenanthroline footprinting of PRP-DNA complexes revealed that PRP protects a 39-base-pair region of PHR1 5{prime} flanking sequence beginning 40 base pairs upstream from the coding sequence. Thus these observations establish that PRP is a damage-responsive repressor of PHR1 transcription.
- OSTI ID:
- 5697974
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:24; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
BIOLOGICAL RADIATION EFFECTS
DNA REPAIR
GENE REGULATION
PROTEINS
BIOCHEMISTRY
METHYL METHANESULFONATE
MOLECULAR BIOLOGY
SACCHAROMYCES CEREVISIAE
TRANSCRIPTION
ULTRAVIOLET RADIATION
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHEMISTRY
ELECTROMAGNETIC RADIATION
ESTERS
EUMYCOTA
FUNGI
MICROORGANISMS
MUTAGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SACCHAROMYCES
SULFONIC ACID ESTERS
YEASTS
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture