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Title: Distance-dependent electron transfer in DNA hairpins

Abstract

The distance dependence of photoinduced electron transfer in duplex DNA was determined for a family of synthetic DNA hairpins in which a stilbene dicarboxamide forms a bridge connecting two oligonucleotide arms. Investigation of the fluorescence and transient absorption spectra of these hairpins established that no photoinduced electron transfer occurs for a hairpin that has six deoxyadenosine-deoxythymidine base pairs. However, the introduction of a single deoxyguanosine-deoxycytidine base pair resulted in distance-dependent fluorescence quenching and the formation of the stilbene anion radical. Kinetic analysis suggests that duplex DNA is somewhat more effective than proteins as a medium for electron transfer but that it does not function as a molecular wire.

Authors:
; ;  [1]
  1. Northwestern Univ., Evanston, IL (United States); and others
Publication Date:
OSTI Identifier:
569263
Resource Type:
Journal Article
Journal Name:
Science
Additional Journal Information:
Journal Volume: 277; Journal Issue: 5326; Other Information: PBD: 1 Aug 1997
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; ELECTRON TRANSFER; DNA

Citation Formats

Lewis, F D, Wu, T, and Zhang, Y. Distance-dependent electron transfer in DNA hairpins. United States: N. p., 1997. Web. doi:10.1126/science.277.5326.673.
Lewis, F D, Wu, T, & Zhang, Y. Distance-dependent electron transfer in DNA hairpins. United States. https://doi.org/10.1126/science.277.5326.673
Lewis, F D, Wu, T, and Zhang, Y. Fri . "Distance-dependent electron transfer in DNA hairpins". United States. https://doi.org/10.1126/science.277.5326.673.
@article{osti_569263,
title = {Distance-dependent electron transfer in DNA hairpins},
author = {Lewis, F D and Wu, T and Zhang, Y},
abstractNote = {The distance dependence of photoinduced electron transfer in duplex DNA was determined for a family of synthetic DNA hairpins in which a stilbene dicarboxamide forms a bridge connecting two oligonucleotide arms. Investigation of the fluorescence and transient absorption spectra of these hairpins established that no photoinduced electron transfer occurs for a hairpin that has six deoxyadenosine-deoxythymidine base pairs. However, the introduction of a single deoxyguanosine-deoxycytidine base pair resulted in distance-dependent fluorescence quenching and the formation of the stilbene anion radical. Kinetic analysis suggests that duplex DNA is somewhat more effective than proteins as a medium for electron transfer but that it does not function as a molecular wire.},
doi = {10.1126/science.277.5326.673},
url = {https://www.osti.gov/biblio/569263}, journal = {Science},
number = 5326,
volume = 277,
place = {United States},
year = {1997},
month = {8}
}