From HeLa cell division to infectious diarrhoea

Journal Article · Sat Sep 01 00:00:00 EDT 1990 · Scanning Microscopy; (USA)

OSTI ID:5682519

Stephen, J; Osborne, M P; Spencer, A J; Warley, A ^[1]

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1. Univ. of Birmingham (England)

Hela S3 cells were grown in suspension both randomly and, synchronously using hydroxyurea which blocks cells at the G1/S interface. Cryosections were prepared, freeze-dried and analyzed by X-ray microanalysis. As cells moved into S and through M phases (Na) and (Cl) increased; both returned to normal levels upon re-entering G1 phase. The Na/K ratio was 1:1 in G1 phase. Infection of HeLa S3 cells in G1 phase with vaccinia virus resulted in no change in intracellular (Na). Infection of neonatal mice with murine rotavirus was localized to villus tip enterocytes and gave rise to diarrhoea which was maximal at 72h post-infection (p.i.). Diarrhoea was preceded by ischemia of villi (18-42h p.i.) and villus shortening (maximal at 42h p.i.), and was also coincident with a dramatic regrowth of villi. At 48h p.i. a proliferative zone of electron lucent cells was observed in villus base regions. Cryosections of infected gut, taken before, during, and after infection, together with corresponding age-matched controls, were freeze-dried and analysed by X-ray microanalysis. At 48h p.i. electron lucent villus base cells were shown to be more hydrated, and, to contain higher levels of both Na and Cl and lower levels of P, S, K and Mg than corresponding control cells. These studies increase confidence in the use of X-ray microanalysis in studying biological systems, provide some insight into the process of cell division, and constitute the basis of a new concept of diarrhoeal secretion.27 references.

• OSTI ID: 5682519
• Journal Information: Scanning Microscopy; (USA), Vol. 4:3; ISSN 0891-7035
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
HELA CELLS
CELL PROLIFERATION
VIRAL DISEASES
DIAGNOSIS
CELL CYCLE
CHEMICAL COMPOSITION
CHLORIDES
DIARRHEA
ELECTRON MICROPROBE ANALYSIS
GASTROINTESTINAL TRACT
MICE
SODIUM
ALKALI METALS
ANIMALS
CHEMICAL ANALYSIS
CHLORINE COMPOUNDS
DIGESTIVE SYSTEM
DISEASES
ELEMENTS
HALIDES
HALOGEN COMPOUNDS
INFECTIOUS DISEASES
MAMMALS
METALS
MICROANALYSIS
RODENTS
SYMPTOMS
VERTEBRATES
550602* - Medicine- External Radiation in Diagnostics- (1980-)