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Title: Changes of ribulose bisphosphate carboxylase/oxygenase content, ribulose bisphosphate concentration, and photosynthetic activity during adaptation of high-CO/sub 2/ grown cells to low-CO/sub 2/ conditions in Chlorella pyrenoidosa

Abstract

Changes of some photosynthetic properties of high-CO/sub 2/ grown cells of Chlorella pyrenoidosa during adaptation to low-CO/sub 2/ conditions have been investigated. The K/sub m/ value of photosynthesis of the high-CO/sub 2/ grown cells for dissolved inorganic carbon was 3.3 millimolar and decreased to 25 to 30 micromolar within 4 hours after transferring to air. In the presence of saturating CO/sub 2/ concentrations the photosynthetic activity of the high-CO/sub 2/ grown cells was 1.5 times as high as that of the low-CO/sub 2/ grown cells. There was a significant rise of the photosynthetic activity during adaptation of the high-CO/sub 2/ grown cells to air, followed by a steady decrease. The activity of ribulose 1,5-bisphosphate carboxylase/oxygenase in both the high and low-CO/sub 2/ grown cells was close to the photosynthetic activity of the cells. The concentration of ribulose 1,5-bisphosphate (RuBP) was higher in the low-CO/sub 2/ adapting and low-CO/sub 2/ grown celsl than in the high-CO/sub 2/ grown cells regardless of the photosynthetic rate. This seems to be due to an increased RuBP regeneration activity during adaptation followed by maintenance of the new higher concentration. The RuBP level always exceeded the concentration of ribulose 1,5-bisphosphate carboxylase/oxygenase RuBP binding sites in bothmore » the high- and low-CO/sub 2/ grown cells at any dissolved inorganic carbon concentration.« less

Authors:
;
Publication Date:
Research Org.:
Queen's Univ., Kingston, Ontario
OSTI Identifier:
5681570
Resource Type:
Journal Article
Journal Name:
Plant Physiol.; (United States)
Additional Journal Information:
Journal Volume: 80:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CARBON DIOXIDE; BIOLOGICAL EFFECTS; CHLORELLA; BIOLOGICAL ADAPTATION; PHOTOSYNTHESIS; CARBON; CARBOXYLASE; CHEMICAL BONDS; ENZYME REACTIVATION; EXPERIMENTAL DATA; OXYGENASES; PLANT CELLS; QUANTITY RATIO; RIBULOSE; SATURATION; ALGAE; CARBOHYDRATES; CARBON COMPOUNDS; CARBON OXIDES; CARBON-CARBON LYASES; CARBOXY-LYASES; CHALCOGENIDES; CHEMICAL REACTIONS; DATA; ELEMENTS; ENZYMES; INFORMATION; KETONES; LYASES; MICROORGANISMS; MONOSACCHARIDES; NONMETALS; NUMERICAL DATA; ORGANIC COMPOUNDS; OXIDES; OXIDOREDUCTASES; OXYGEN COMPOUNDS; PENTOSES; PHOTOCHEMICAL REACTIONS; PLANTS; SACCHARIDES; SYNTHESIS; UNICELLULAR ALGAE; 550500* - Metabolism; 560303 - Chemicals Metabolism & Toxicology- Plants- (-1987)

Citation Formats

Yokota, A, and Canvin, D T. Changes of ribulose bisphosphate carboxylase/oxygenase content, ribulose bisphosphate concentration, and photosynthetic activity during adaptation of high-CO/sub 2/ grown cells to low-CO/sub 2/ conditions in Chlorella pyrenoidosa. United States: N. p., 1986. Web.
Yokota, A, & Canvin, D T. Changes of ribulose bisphosphate carboxylase/oxygenase content, ribulose bisphosphate concentration, and photosynthetic activity during adaptation of high-CO/sub 2/ grown cells to low-CO/sub 2/ conditions in Chlorella pyrenoidosa. United States.
Yokota, A, and Canvin, D T. Sat . "Changes of ribulose bisphosphate carboxylase/oxygenase content, ribulose bisphosphate concentration, and photosynthetic activity during adaptation of high-CO/sub 2/ grown cells to low-CO/sub 2/ conditions in Chlorella pyrenoidosa". United States.
@article{osti_5681570,
title = {Changes of ribulose bisphosphate carboxylase/oxygenase content, ribulose bisphosphate concentration, and photosynthetic activity during adaptation of high-CO/sub 2/ grown cells to low-CO/sub 2/ conditions in Chlorella pyrenoidosa},
author = {Yokota, A and Canvin, D T},
abstractNote = {Changes of some photosynthetic properties of high-CO/sub 2/ grown cells of Chlorella pyrenoidosa during adaptation to low-CO/sub 2/ conditions have been investigated. The K/sub m/ value of photosynthesis of the high-CO/sub 2/ grown cells for dissolved inorganic carbon was 3.3 millimolar and decreased to 25 to 30 micromolar within 4 hours after transferring to air. In the presence of saturating CO/sub 2/ concentrations the photosynthetic activity of the high-CO/sub 2/ grown cells was 1.5 times as high as that of the low-CO/sub 2/ grown cells. There was a significant rise of the photosynthetic activity during adaptation of the high-CO/sub 2/ grown cells to air, followed by a steady decrease. The activity of ribulose 1,5-bisphosphate carboxylase/oxygenase in both the high and low-CO/sub 2/ grown cells was close to the photosynthetic activity of the cells. The concentration of ribulose 1,5-bisphosphate (RuBP) was higher in the low-CO/sub 2/ adapting and low-CO/sub 2/ grown celsl than in the high-CO/sub 2/ grown cells regardless of the photosynthetic rate. This seems to be due to an increased RuBP regeneration activity during adaptation followed by maintenance of the new higher concentration. The RuBP level always exceeded the concentration of ribulose 1,5-bisphosphate carboxylase/oxygenase RuBP binding sites in both the high- and low-CO/sub 2/ grown cells at any dissolved inorganic carbon concentration.},
doi = {},
url = {https://www.osti.gov/biblio/5681570}, journal = {Plant Physiol.; (United States)},
number = ,
volume = 80:2,
place = {United States},
year = {1986},
month = {2}
}