Effect of halothane on the natural-abundance 13C NMR spectra of excised rat brain

Journal Article · Tue Dec 31 00:00:00 EST 1985 · Biochemistry; (United States)

OSTI ID:5674101

Barany, M; Chang, Y C; Arus, C

Halothane increases the intensity of the 30.5- and 129-ppm resonances in 13C nuclear magnetic resonance spectra of excised rat brain, of phospholipid vesicles prepared from chloroform-methanol extract of rat brain, and of brain excised from rats anesthetized with halothane. The 13C spin-lattice relaxation times of the 30.5- and 129-ppm resonances are increased in excised brain, or phospholipid vesicles, upon addition of halothane, and they are also increased in brain excised from rats anesthetized with halothane. Excised brain and its membrane-rich subcellular fractions interact with (14C)halothane reversibly. The interaction is virtually abolished when the phospholipids are extracted from the brain. The (14C)halothane content of the brain membranes is correlated with the halothane-induced increase in the integral of the 129-ppm resonance. From this correlation and from the phospholipid content of the membranes, a halothane concentration of 3.34 mM and a partition of 0.057 mol of halothane/mol of phospholipid may be calculated in the brain of anesthetized rats.

• Research Organization: Univ. of Illinois, Chicago
• OSTI ID: 5674101
• Journal Information: Biochemistry; (United States), Vol. 27
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BRAIN
NMR SPECTRA
HALOGENATED ALIPHATIC HYDROCARBONS
BIOLOGICAL EFFECTS
CARBON 13
CARBON 14 COMPOUNDS
LIPOSOMES
NUCLEAR MAGNETIC RESONANCE
PHOSPHOLIPIDS
RATS
TRACER TECHNIQUES
ANIMALS
BODY
CARBON ISOTOPES
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
ESTERS
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPIDS
MAGNETIC RESONANCE
MAMMALS
NERVOUS SYSTEM
NUCLEI
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
ORGANS
RESONANCE
RODENTS
SPECTRA
STABLE ISOTOPES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)