Action of DTPA on hepatic plutonium. III. Evidence for a direct chelation mechanism for DTPA-induced excretion of monomeric plutonium into rat bile. [/sup 239/Pu]
Trisodium calcium diethylenetriaminepenta(2-/sup 14/C)acetic acid ((/sup 14/C)DTPA, 0.25 nmole/kg, 0.210 mCi/mmole) was administered via the jugular vein to three rats whose bile ducts and urinary bladders were cannulated. Bile and urine were collected for 24 h after injection, and tissues and excreta were then analyzed for /sup 14/C content. (/sup 14/C)DTPA was identified in the bile samples by cochromatography with unlabeled DTPA on an anion-exchange column system. Data from this experiment demonstrated the existence of a minor excretion pathway for DTPA into rat bile, accounting for 0.12% of the injected dose by 24 h after administration. Bile was collected for 24 h following the administration of unlabeled Na/sub 3/(CaDTPA) (0.25 mmole/kg, iv) to a rat which had received /sup 239/Pu(IV)-citrate (5.7 ..mu..Ci/kg, iv) 24 h prior to DTPA. Bile samples containing Pu were analyzed using anion-exchange column chromatography. Eighty to ninety percent of the Pu present in bile chromatographed as a single peak in the position of the Pu-DTPA complex. Cochromatograhy of the 0- to 6-h bile sample with a /sup 239/Pu-(/sup 14/C)DTPA complex revealed that the Pu appearing in bile following DTPA treatment comigrated with the /sup 239/Pu-(/sup 14/C)DTPA complex. These data provide strong evidence that, in the rat, DTPA acts to remove hepatic deposits of monomeric Pu by formation of the Pu-DTPA complex followed by excretion of the complex into bile.
- Research Organization:
- Argonne National Lab., IL
- OSTI ID:
- 5673887
- Journal Information:
- Radiat. Res.; (United States), Vol. 80:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DTPA
BIOLOGICAL EFFECTS
LIVER
RADIONUCLIDE KINETICS
PLUTONIUM 239
EXCRETION
PLUTONIUM COMPLEXES
BILE
BILIARY TRACT
BIOLOGICAL LOCALIZATION
BLADDER
CARBON 14 COMPOUNDS
EXPERIMENTAL DATA
INTRAVENOUS INJECTION
ION EXCHANGE CHROMATOGRAPHY
ISOLATED VALUES
RATS
RENAL CLEARANCE
RETENTION FUNCTIONS
TRACER TECHNIQUES
ACTINIDE COMPLEXES
ACTINIDE ISOTOPES
ACTINIDE NUCLEI
ALPHA DECAY RADIOISOTOPES
AMINO ACIDS
ANIMALS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CHELATING AGENTS
CHROMATOGRAPHY
CLEARANCE
COMPLEXES
DATA
DATA FORMS
DIGESTIVE SYSTEM
DRUGS
EVEN-ODD NUCLEI
GLANDS
HEAVY NUCLEI
INFORMATION
INJECTION
INTAKE
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
NUCLEI
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PLUTONIUM ISOTOPES
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RESPONSE MODIFYING FACTORS
RODENTS
SEPARATION PROCESSES
TRANSURANIUM COMPLEXES
URINARY TRACT
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560172* - Radiation Effects- Nuclide Kinetics & Toxicology- Animals- (-1987)