Strengthening of the DNA-protein complex during stationary phase aging of cell cultures
The possibility of accumulation of cross-linkages in the DNA-protein complex was studied during stationary phase aging of cells in culture. Chinese hamster cells were used in the experiments, along with human fibroblasts. /sup 3/H-thymidine, /sup 14/C-valine, and /sup 14/C-leucine were added to the medium. The quantity of protein firmly bound with DNA was judged from the value of the coefficient /sup 14/C//sup 3/H determined with allowance for penetration of counting from the /sup 14/C-channel into the /sup 3/H-channel. The authors maintain that the results presented in this paper provide further evidence of the value of stationary phase cell cultures for the study of the mechanisms of aging and also of some of the general principles underlying hereditary pathology.
- Research Organization:
- Research Institute of Physicochemical Medicine, Moscow, USSR
- OSTI ID:
- 5669479
- Journal Information:
- Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Vol. 101:4; Other Information: Translated from Byulleten' Ehksperimental'noj Biologii i Meditsiny; 101: No. 4, 416-418(Apr 1986)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHO CELLS
AGING
DNA
BINDING ENERGY
CROSS-LINKING
TRACER TECHNIQUES
EMBRYONIC CELLS
FIBROBLASTS
PROTEINS
BIOLOGICAL REPAIR
CARBON 14 COMPOUNDS
CELL CULTURES
COMPLEXOMETRY
GENETICS
LABELLING
LEUCINE
MAN
PATIENTS
RADIOACTIVITY
SCINTILLATION COUNTING
SISTER CHROMATID EXCHANGES
THYMIDINE
TRITIUM COMPOUNDS
VALINE
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL RECOVERY
BIOLOGY
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
COUNTING TECHNIQUES
ENERGY
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MUTATIONS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYMERIZATION
PRIMATES
PYRIMIDINES
RECOVERY
REPAIR
RIBOSIDES
SOMATIC CELLS
VERTEBRATES
550301* - Cytology- Tracer Techniques
550401 - Genetics- Tracer Techniques