Slit-scan flow cytometer for recording simultaneous waveforms

Journal Article · Wed May 01 00:00:00 EDT 1985 · Rev. Sci. Instrum.; (United States)

DOI:https://doi.org/10.1063/1.1138207· OSTI ID:5668096

Johnston, R G; Bartholdi, M F; Hiebert, R D; Parson, J D; Cram, L S

We have developed a slit-scan flow cytometer for recording the transient, simultaneous light scattering and fluorescence waveforms from individual metaphase chromosomes flowing through a focused laser beam. The two waveforms are recorded on a single-memory waveform recorder by delaying one of the waveforms and switching between them. The light scattering and fluorescence waveforms contain complementary information on chromosome structure. The delay and switching electronics developed here may be useful in other contexts.

• Research Organization: Experimental Pathology Group, Mail Stop M888, Los Alamos National Laboratory, Los Alamos, New Mexico 87545
• OSTI ID: 5668096
• Journal Information: Rev. Sci. Instrum.; (United States), Vol. 56:5
• Country of Publication: United States
• Language: English

Related Subjects

47 OTHER INSTRUMENTATION
CHROMOSOMES
FLUORESCENCE
LIGHT SCATTERING
WAVE FORMS
RECORDING SYSTEMS
ELECTRONIC CIRCUITS
ELECTRONIC EQUIPMENT
LASER RADIATION
MEASURING METHODS
ELECTROMAGNETIC RADIATION
EQUIPMENT
LUMINESCENCE
RADIATIONS
SCATTERING
440300* - Miscellaneous Instruments- (-1989)