In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy

doi:https://doi.org/10.1126/science.6353573

Title: In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy

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Abstract

An intracellular coenzyme has been observed by carbon-13 nuclear magnetic resonance spectroscopy. The pyridine nucleotides in Escherichia coli were specifically labeled with carbon-13 from the biosynthetic precursor, nicotinic acid. The intracellular redox status and metabolic transformations of the pyridine nucleotides were examined under a variety of conditions. A highly reduced nicotinamide adenine dinucleotide pool was observed under anaerobic conditions only in cells that were cultured aerobically on glycerol.

Authors:: Unkefer, C J; Blazer, R M; London, R E

Publication Date:: 1983-10-07

Research Org.:: Los Alamos National Lab., NM

OSTI Identifier:: 5663280

Resource Type:: Journal Article

Journal Name:: Science (Washington, D.C.); (United States)

Additional Journal Information:: Journal Volume: 222

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; CARBON 13; NUCLEAR MAGNETIC RESONANCE; NAD; LABELLED COMPOUNDS; LABELLED POOL TECHNIQUES; METABOLISM; REDUCTION; NADP; ESCHERICHIA COLI; IN VIVO; REDOX REACTIONS; BACTERIA; CARBON ISOTOPES; CHEMICAL REACTIONS; COENZYMES; EVEN-ODD NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MAGNETIC RESONANCE; MICROORGANISMS; NUCLEI; NUCLEOTIDES; ORGANIC COMPOUNDS; RESONANCE; STABLE ISOTOPES; TRACER TECHNIQUES; 550200* - Biochemistry

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                    Unkefer, C J, Blazer, R M, and London, R E. In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy.  United States: N. p., 1983. 
        Web.  doi:10.1126/science.6353573.

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                    Unkefer, C J, Blazer, R M, & London, R E. In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy.  United States.  https://doi.org/10.1126/science.6353573

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                    Unkefer, C J, Blazer, R M, and London, R E. Fri .  
        "In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy".  United States.  https://doi.org/10.1126/science.6353573.

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@article{osti_5663280,

  title        = {In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy},

  author       = {Unkefer, C J and Blazer, R M and London, R E},

  abstractNote = {An intracellular coenzyme has been observed by carbon-13 nuclear magnetic resonance spectroscopy. The pyridine nucleotides in Escherichia coli were specifically labeled with carbon-13 from the biosynthetic precursor, nicotinic acid. The intracellular redox status and metabolic transformations of the pyridine nucleotides were examined under a variety of conditions. A highly reduced nicotinamide adenine dinucleotide pool was observed under anaerobic conditions only in cells that were cultured aerobically on glycerol.},

  doi          = {10.1126/science.6353573},

  url          = {https://www.osti.gov/biblio/5663280},
  journal      = {Science (Washington, D.C.); (United States)},
number       = ,

  volume       = 222,

  place        = {United States},

  year         = {1983},

  month        = {10}

}

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In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy

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