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Title: In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy

Abstract

An intracellular coenzyme has been observed by carbon-13 nuclear magnetic resonance spectroscopy. The pyridine nucleotides in Escherichia coli were specifically labeled with carbon-13 from the biosynthetic precursor, nicotinic acid. The intracellular redox status and metabolic transformations of the pyridine nucleotides were examined under a variety of conditions. A highly reduced nicotinamide adenine dinucleotide pool was observed under anaerobic conditions only in cells that were cultured aerobically on glycerol.

Authors:
; ;
Publication Date:
Research Org.:
Los Alamos National Lab., NM
OSTI Identifier:
5663280
Resource Type:
Journal Article
Journal Name:
Science (Washington, D.C.); (United States)
Additional Journal Information:
Journal Volume: 222
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CARBON 13; NUCLEAR MAGNETIC RESONANCE; NAD; LABELLED COMPOUNDS; LABELLED POOL TECHNIQUES; METABOLISM; REDUCTION; NADP; ESCHERICHIA COLI; IN VIVO; REDOX REACTIONS; BACTERIA; CARBON ISOTOPES; CHEMICAL REACTIONS; COENZYMES; EVEN-ODD NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MAGNETIC RESONANCE; MICROORGANISMS; NUCLEI; NUCLEOTIDES; ORGANIC COMPOUNDS; RESONANCE; STABLE ISOTOPES; TRACER TECHNIQUES; 550200* - Biochemistry

Citation Formats

Unkefer, C J, Blazer, R M, and London, R E. In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy. United States: N. p., 1983. Web. doi:10.1126/science.6353573.
Unkefer, C J, Blazer, R M, & London, R E. In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy. United States. https://doi.org/10.1126/science.6353573
Unkefer, C J, Blazer, R M, and London, R E. Fri . "In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy". United States. https://doi.org/10.1126/science.6353573.
@article{osti_5663280,
title = {In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy},
author = {Unkefer, C J and Blazer, R M and London, R E},
abstractNote = {An intracellular coenzyme has been observed by carbon-13 nuclear magnetic resonance spectroscopy. The pyridine nucleotides in Escherichia coli were specifically labeled with carbon-13 from the biosynthetic precursor, nicotinic acid. The intracellular redox status and metabolic transformations of the pyridine nucleotides were examined under a variety of conditions. A highly reduced nicotinamide adenine dinucleotide pool was observed under anaerobic conditions only in cells that were cultured aerobically on glycerol.},
doi = {10.1126/science.6353573},
url = {https://www.osti.gov/biblio/5663280}, journal = {Science (Washington, D.C.); (United States)},
number = ,
volume = 222,
place = {United States},
year = {1983},
month = {10}
}