Metabolism of (/sup 3/H) 5 alpha-androstane-3 alpha, 17 beta-diol by cultures of isolated rat sertoli cells and the effect of LH and FSH

Journal Article · Sun Apr 01 00:00:00 EST 1984 · Steroids; (United States)

DOI:https://doi.org/10.1016/0039-128X(84)90019-9· OSTI ID:5655741

Tcholakian, R K; Berkowitz, A S; Newaz, S N

Sertoli cells from immature rats metabolized (/sup 3/H) 5 alpha-androstane-3 alpha, 17 beta-diol to (/sup 3/H) 5 alpha-androstane-3 alpha, 16 alpha, 17 beta-triol and (/sup 3/H) 3 alpha-hydroxy-5 alpha-androstan-17-one. This is the first report of 16 alpha-hydroxylation of 5 alpha-reduced androgens in the testis. FSH significantly stimulated 16 alpha-hydroxylation while LH significantly decreased this activity. 3 alpha-Hydroxy-5 alpha-androstan-17-one was the major metabolite formed and its production was significantly increased in the presence of both LH and FSH, although FSH stimulation was significantly more than LH. The possible role of 16 alpha-hydroxylase in androgen metabolism by immature rat Sertoli cells is discussed.

• Research Organization: Univ. of Texas Medical Schoo, Houston
• OSTI ID: 5655741
• Journal Information: Steroids; (United States), Vol. 43:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANDROGENS
HYDROXYLATION
ANDROSTANES
METABOLISM
FSH
BIOLOGICAL EFFECTS
LH
CELL CULTURES
GLYCOLS
HYDROXYLASES
METABOLITES
RATS
TESTES
ALCOHOLS
ANIMALS
BODY
ENZYMES
GONADOTROPINS
GONADS
HORMONES
HYDROXY COMPOUNDS
MALE GENITALS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEPTIDE HORMONES
PITUITARY HORMONES
RODENTS
STEROID HORMONES
STEROIDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques