The biological effects of ozone on representative members of five groups of animal viruses
In an effort to establish the biological relevance of the reactions of ozone with soluble proteins and lipid bilayer membrane systems, representative viruses from five major virus groups were exposed to moderate concentrations of ozone. The virus suspensions were exposed at 37/sup 0/C to 0.00, 0.16, and 0.64 ppm ozone in the gas phase. The ozone reacted with the virus suspensions as a thin film of fluid on the surface of a rotating culture bottle as the gas was drawn through the bottle at a flow rate of 2 liters/min. The three enveloped viruses tested exhibited different susceptibilities to ozone inactivation which correlated with their thermolability in the absence of ozone. The order of susceptibility to ozone inactivation of the enveloped viruses was vesicular stomatitis virus (VSV) (Rhabdoviridae) > influenza A virus (WSN strain) (Orthomyxoviridae) > infectious bovine rhinotracheitis virus (IBRV) (Herpesviridae). The inactivation reactions of the enveloped viruses with ozone showed pseudo-first-order kinetics. A simple reaction model was used to derive a reaction rate expression from which rate constrants and reaction stoichiometry were estimated. In contrast to the enveloped viruses, the two nonenveloped viruses examined were relatively resistant to ozone inactivation. Polio virus type I (Picornaviridae) was found to be completely resistant to ozone inactivation after 60 hr exposure to either ozone concentration, while infectious canine hepatitis virus (Adenoviridae) showed only slight inactivation after exposure to 0.64 ppm ozone for 66 hr. The significance of these results with regard to the reactions of ozone with cell membranes and other components is discussed.
- Research Organization:
- Univ. of California, Davis
- OSTI ID:
- 5653911
- Journal Information:
- Environ. Res.; (United States), Vol. 27:2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Virucidal levels of ozone induce hemolysis and hemoglobin degradation
Inactivation of viruses in platelet suspensions that retain their in vitro characteristics: Comparison of psoralen-ultraviolet A and merocyanine 540-visible light methods
Related Subjects
OZONE
BIOLOGICAL EFFECTS
VIRUSES
INACTIVATION
BIOCHEMICAL REACTION KINETICS
CELL MEMBRANES
CELL CONSTITUENTS
KINETICS
MEMBRANES
MICROORGANISMS
PARASITES
REACTION KINETICS
560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)